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Direct cGMP ELISA kit

ADI-900-014

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Most sensitive (0.025 pmol/ml) colorimetric kit for intracellular cGMP quantification.

Highly sensitive ELISA with an optional acetylation protocol which increases sensitivity 10-fold (0.025 pmol/ml)
Trusted and widely cited in peer-reviewed publications
Higher throughput format with results for up to 39 samples in duplicate
Simple, efficient, and well-established sample handling protocols
Reliable and consistent lot-to-lot performance

This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.

Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.

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Product Details

Alternative Name	cyclic GMP
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of cGMP in cell lysates and tissue from any species. Cited sample types include cerebral spinal fluid and microdialysate.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
Crossreactivity	cyclic GMP (100%) and <0.001% GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP
Sensitivity	Non-acetylated format: 0.604 pmol/mlAcetylated 2-hour format: 0.059 pmol/mlAcetylated overnight format: 0.025 pmol/ml Range: (non-acetylated): 0.8 – 500 pmol/mlRange: (acetylated): 0.08 – 50 pmol/ml)
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Use/Stability	Store all components at +4°C, except standard and conjugate at -20°C.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Lipocalin-2 Restores Soluble Guanylyl Cyclase-Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice: Zhao, L., Xu, M., et al.; Acta Physiol. (Oxf.) 241, e70077 (2025), Abstract
The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice: de Oliveira do Rêgo, A. G., Maccari, S., et al.; Life Sci. Alliance 8, e202403094 (2025), Reactant(s): Mouse, Abstract
The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling: C. Carlantoni, et al.; Cell 59, 308 (2024), Abstract
Molsidomine provides neuroprotection against vincristine-induced peripheral neurotoxicity through soluble guanylyl cyclase activation: Utkina-Sosunova, I., Chiorazzi, A., et al.; Sci. Rep. 14, 19341 (2024), Abstract
Protein-based vaccine candidate MecVax broadly protects against enterotoxigenic Escherichia coli intestinal colonization in a rabbit model: I. Upadhyay, et al.; Infect. Immun. 91, e0027223 (2023), Abstract
Synergistic Effects of Resistive Breathing on Endotoxin-Induced Lung Injury in Mice: D. Toumpanakis, et al.; Int. J. Chron. Obstruct. Pulmon. Dis. 18, 2321 (2023), Abstract
Anti-Toxin Responses to Natural Enterotoxigenic Escherichia coli (ETEC) Infection in Adults and Children in Bangladesh: P. Girardi, et al.; Microorganisms 11, 2524 (2023), Abstract
Magnetic resonance imaging detects white adipose tissue beiging in mice following PDE10A inhibitor treatment: M.R. Tomaszewski, et al.; J. Lipid Res. 64, 100408 (2023), Abstract
Effects of a Soluble Guanylate Cyclase Stimulator Riociguat on Contractility of Isolated Pulmonary Artery and Hemodynamics of U46619-Induced Pulmonary Hypertension in Dogs: S. Kameshima, et al.; Vet. Sci. 10, 159 (2023), Abstract
The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling: Carlantoni, C., Liekfeld, L., et al.; bioRxiv , (2023)

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