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Direct cAMP ELISA kit

ADI-900-066A

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Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.

Highly sensitive with an optional acetylation protocol which increases sensitivity >10-fold
High throughput format, results for up to 39 samples in duplicate in just 3 hours
Flexible assay, species independent
Easy-to-use pre-coated plate and liquid color-coded reagents reduce errors
Efficient and well-established sample handling protocols
Widely cited in peer-reviewed publications

This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.

Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

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Direct cAMP ELISA kit Standard curve - Acetylated — Typical standard curve used to calculate cAMP concentrations for Acetylated assay format.

Typical standard curve used to calculate cAMP concentrations for Non-acetylated assay format.

Product Details

Alternative Name	cyclic AMP
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
Crossreactivity	cyclic AMP (100%)AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)
Sensitivity	Non-acetylated: 1.18 pmol/ml (range 0.78 – 200 pmol/ml)Acetylated: 0.006 pmol/ml (range 0.078 – 20 pmol/ml)
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Use/Stability	Store all components at +4°C, except standard and conjugate at -20°C.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Begomovirus capsid proteins interact with cyclic adenosine monophosphate (cAMP)-specific phosphodiesterase of its whitefly vector and modulate virus retention within its vector: Ghosh, S., Mondal, B., et al.; J. Virol. 99, e0217224 (2025), Abstract
Cyclic AMP Rescue of Motility in Sperm Devoid of Soluble Adenylyl Cyclase: Ayoub, S., Rivera Sanchez, N. D. R., et al.; Int. J. Mol. Sci. 26, 1489 (2025), Abstract
Two unreported lecanoric acid derivatives isolated from Chaetomium globosum KM651986 and their anti-diabetic effect: R.A. Hussein, et al.; Sci. Rep. 15, 23554 (2025), Abstract — Full Text
Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activation: Shao, J., Kwon, J., et al.; Nat. Commun. 16, 6232 (2025), Reactant(s): Human, Abstract
The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice: de Oliveira do Rêgo, A. G., Maccari, S., et al.; Life Sci. Alliance 8, e202403094 (2025), Reactant(s): Mouse, Abstract
Loss-of-function of GNAL dystonia gene impairs striatal dopamine receptors-mediated adenylyl cyclase/ cyclic AMP signaling pathway: I.E. Atiallah, et al.; Neurobiol. Dis. 191, 106403 (2024), Abstract
Protein SUMOylation promotes cAMP-independent EPAC1 activation: W. Yang, et al.; bioRxiv , (2024), Abstract
The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling: C. Carlantoni, et al.; Cell 59, 308 (2024), Abstract
Chronic Stress-Induced Elevation of Melanin-Concentrating Hormone in the Locus Coeruleus Inhibits Norepinephrine Production and Associated With Depression-Like Behaviors in Rats: N. Kurban, et al.; Int. J. Neuropsychopharmacol. 27, pyad069 (2024), Abstract
Distinct binding conformations of epinephrine with α- and β-adrenergic receptors: J.S. Lou, et al.; Exp. Mol. Med. 56, 1952 (2024), Abstract

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