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Direct 8-iso-PGF2α ELISA kit

ADI-900-091

33 citations

ELISA kit
  • ADI-900-091   —   96 wells
    $502.00
  • Non-radioactive measurement of isoprostane in serum and plasma
  • Flexible options, either overnight or same day results
  • High sensitivity, measuring as little as 40 pg/ml
  • High throughput format with results for up to 39 samples in duplicate in less than 3 hours

The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. The is a colorimetric competitive immunoassay kit for the quantitative determination of 8-iso-Prostaglandin F in biological fluids such as plasma, serum and tissue. To measure isoprostane in urine or culture supernatants, use the 8-iso-PGF EIA Kit.

8-iso-PGF is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF has been shown to circulate in plasma and is excreted in urine.

Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.

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Regulatory Status

RUO – Research Use Only