Direct 8-iso-PGF_2α ELISA kit

• Non-radioactive measurement of isoprostane in serum and plasma
• Flexible options, either overnight or same day results
• High sensitivity, measuring as little as 40 pg/ml
• High throughput format with results for up to 39 samples in duplicate in less than 3 hours

The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. The is a colorimetric competitive immunoassay kit for the quantitative determination of 8-iso-Prostaglandin F_2α in biological fluids such as plasma, serum and tissue. To measure isoprostane in urine or culture supernatants, use the 8-iso-PGF_2α EIA Kit.

8-iso-PGF_2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF_2α has been shown to circulate in plasma and is excreted in urine.

Prostaglandin F_2α (PGF_2α) is formed in a variety of cells from PGH₂, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF_2α is often viewed as an antagonist to PGE₂ due to their opposing effects on various tissues. PGF_2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF_2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF_2α have also been associated with preeclampsia.