DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environmentsensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. DiIC12(3) is a lipophilic neuronal tracer that is commonly used for labeling neuronal projections as well as lipid bilayers in other cell types, particularly endothelial cells. DiIC12(3) exhibits low toxicity and minimally effects cell viability and is tolerable in culture for several days, making it suitable across a variety of applications. Wavelength Maxima: Excitation 549nm, Emission 565nm
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