Dihydrorhodamine 123 is a widely utilized probe for detection of intracellular reactive oxygen species (ROS) such as peroxide, hypochlorous acid and peroxynitrite. It is readily oxidized into rhodamine 123, which exhibits a spectral profile similar to that of FITC. In combination with other fluorescent probes (such as surface receptor-targeted fluorescent antibodies, the cell viability probe propidium iodide, or fluorescent calcium indicators) dihydrorhodamine 123 can be used for multiparametric cell measurements. Wavelength Maxima: Excitation 507nm, Emission 529nm
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