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DHEA ELISA kit

ADI-901-093

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Highly sensitive ELISA, measuring DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.

Specific with low reactivity to structurally related molecules
Highly reproducible with less than 9% inter- and intra-assay variation
Highly sensitive, detecting low levels of DHEA
Fast results for up to 37 samples in duplicate in 5 hours
Save with bulk kit package!

The DHEA ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. This kit has low reactivity to structurally related molecules and offers sensitive measurement of DHEA in a variety of sample types.

Dehydroepiandrosterone (DHEA) is a 17-ketosteroid produced by side chain cleavage of 17-hydroxypregnenolone. DHEA is reversibly converted to its sulfate ester (DHEA-S) and Androstendiol. The human adrenal glands secrete both DHEA and DHEA-S which in turn serve as the precursors for both estrogenic and androgenic steroids. DHEA has been linked to a number of physiological conditions including depression, systemic lupus erythematosus, and rheumatoid arthritis.

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Product Details

Alternative Name	Dehydroepiandrosterone
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.
Assay Time	5 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Crossreactivity	DHEA (100%), DHEA sulfate (30.0%), Androstenedione (0.73%), Androsterone (0.29%), Pregnenolone (0.28%), Testosterone (0.10%), Progesterone (0.06%), Estradiol (0.04%) and <0.02%: 20-hydroxycholesterol, Androsterone sulphate, DHEA sodium sulphate, 17-hydroxyepipregnanolone, 19-hydroxyandrostendione, 17-hydroxyprogesterone, Cortisol
Sensitivity	2.9 pg/ml (range 12.21 – 50,000 pg/ml)
Species Reactivity	Species independent
Technical Info / Product Notes	Cited samples: Wildlife Endocrinology: Cited Sample Types
Wavelength	405 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women: Cerro, E. D., Félix, J., et al.; Biomolecules 15, 739 (2025), Abstract
Associations of prenatal and childhood Pb exposure with allostatic load in adolescence: Findings from the ELEMENT cohort study: Halabicky, O. M., Tellez-Rojo, M. M., et al.; Environ. Res. 235, 116647 (2023), Abstract
Adolescence is characterized by more sedentary behaviour and less physical activity even among highly active forager-farmers: Caldwell, A. E., Cummings, D., et al.; Proc. Biol. Sci. 290, 20231764 (2023), Abstract
Adolescence is characterized by more sedentary behavior and less physical activity even among highly active forager-farmers: Caldwell, A. E., Cummings, D. K., et al.; medRxiv , (2023)
Divergent neural and endocrine responses in wild-caught and laboratory-bred Rattus norvegicus: J. Jacob, et al.; Behav. Brain Res. 432, 113978 (2022), Abstract
Adrenal MT1 melatonin receptor expression is linked with seasonal variation in social behavior in male Siberian hamsters: Munley, K. M., Dutta, S., et al.; Horm. Behav. 138, 105099 (2022), Abstract
Influence of Effort-based Reward Training on Neuroadaptive Cognitive Responses: Implications for Preclinical Behavioral Approaches for Depressive Symptoms: E. Ploppert, et al.; Neuroscience 500, 63 (2022), Abstract
Disrupted development from head to tail: Pervasive effects of postnatal restricted resources on neurobiological, behavioral, and morphometric outcomes: M.H. Kent, et al.; Front. Behav. Neurosci. 16, 910056 (2022), Abstract
Improvement of several stress response and sleep quality hormones in men and women after sleeping in a bed that protects against electromagnetic fields: E.D.D. Cerro, et al.; Environ. Health 21, 72 (2022), Abstract
Novel regulation mechanism of adrenal cortisol and DHEA biosynthesis via the endogen ERAD inhibitor small VCP-interacting protein: R. Iihan, et al.; Sci. Rep. 12, 869 (2022), Abstract