DAPI is a fluorescent dye that binds nucleic acids and demonstrates a high affinity for DNA. It is used extensively in fluorescence microscopy, flow cytometry and microplate assays. DAPI is cell permeable and is readily used for detecting both live, dead and fixed cells. DAPI emits in the blue spectral range and is ideal for multiplexed assays inclusive of green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Aside from labeling cell nuclei, DAPI is also used for the detection of mycoplasma or viral DNA in cell cultures. Wavelength Maxima: Excitation 358nm, Emission 461nm
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RUO – Research Use Only |
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