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DAF-2 DA (cell permeable)

ALX-620-056

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Fluorescent probe for NO detection. Cell permeable.

Cell permeable analog of DAF-2 (Prod. No. ALX-620-052) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm).

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Product Details

Alternative Name	4,5-Diaminofluorescein . diacetate
Appearance	Liquid.
CAS	205391-02-2
Formula	C₂₄H₁₈N₂O₇
Formulation	Dissolved in 0.45ml DMSO.
MW	446.4
Purity	≥98% (HPLC)
Technical Info / Product Notes	Replacement for ADI-906-042

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
Handling	Protect from light. Keep under inert gas. Hygroscopic. After opening, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

High-Throughput Screening and Proteomic Characterization of Compounds Targeting Myeloid-Derived Suppressor Cells: Krumm, J., Petrova, E., et al.; Mol. Cell. Proteomics 22, 100632 (2023), Abstract
Identification of inhibitors of myeloid-derived suppressor cells activity through phenotypic chemical screening: Schröder, M., Loos, S., et al.; Oncoimmunology 6, e1258503 (2017), Abstract
Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice: A.M. Gamez-Mendez, et al.; PLoS One 10, e0138609 (2015), Application(s): In situ nitric oxide detection in mice, Abstract — Full Text
Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration: Guterbaum, T. J., Braunstein, T. H., et al.; J. Vasc. Res. 50, 410 (2013), Abstract
BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube: A. Traister, et al.; Cell Death Differ. 11, 832 (2004), Abstract
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem. 277, 25823 (2002), Abstract — Full Text
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001), Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001), Abstract
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001), Abstract
Fluroescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)

Related Products

DAF-2

ALX-620-052

Fluorescent probe for NO detection

11 citations

Alternative Name	4,5-Diaminofluorescein
CAS	205391-01-1
Purity	≥98% (HPLC)

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DAF-2 DA (cell permeable)

ALX-620-056

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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