DAF-2

ALX-620-052

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Fluorescent probe for NO detection

Highly sensitive fluorescent probe for the real-time detection of nitric oxide (NO) in vivo. In the cell, the product reacts rapidly with NO in the presence of O₂ to form the highly fluorescent compound triazolofluorescein (DAF-2T, Ex 495nm; Em 515nm). Not for sale in Japan.

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in the presence of NO and O2 — **Figure:** The cell-permeable diacetate derivative, DAF-2 DA (Prod. No. ALX-620-056), is used to load cells. Subsequent hydrolysis by cytosolic esterases releases DAF-2 (Prod. No. ALX-620-052)

Product Details

Alternative Name	4,5-Diaminofluorescein
Appearance	Pale yellow liquid.
CAS	205391-01-1
Formula	C₂₀H₁₆N₂O₅
Formulation	Dissolved in 0.55ml DMSO.
MW	364.3
Purity	≥98% (HPLC)

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
Handling	Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Hypoxic and nitrosative stress conditions modulate expression of myoglobin genes in a carcinogenic hepatobiliary trematode, Clonorchis sinensis: S.H.Kim, et al.; PLoS Negl. Trop. Dis. 15, e0009811 (2021), Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001), Abstract
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001), Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001), Abstract
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000), Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000), Abstract
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998), Abstract
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998), Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998), Abstract

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DAF-2

ALX-620-052

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