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This antibody is covered by our Worry-Free Guarantee.
Product Details
Alternative Name |
CK8 |
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Application |
IHC (PS), WB |
Clone |
35βH11 |
Formulation |
Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA. |
Host |
Mouse |
Immunogen |
Triton/High salt-insoluble material from the human hepatocellular carcinoma cell line Hep3B. |
Isotype |
IgM |
Purity Detail |
Affinity purified. |
Recommendation Dilutions/Conditions |
Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. Choose a good quality enzyme-linked anti-mouse IgM antibody for detection)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from hybridoma tissue culture supernatant. |
Species Reactivity |
Human |
Specificity |
35βH11 is specific for “low molecular weight” cytokeratin 8 (52.5 kDa) characteristically found in “simple” epithelium (e.g., hepatocytes, pancreatic acinar cells, absorptive cells of the GI tract, etc.), ductal structures (e.g., of liver and breast), but not in squamous epithelium. The antibody stains carcinomas such as ductal carcinoma of the breast, liver and pancreas, adenocarcinoma (ovary, lung, endometrium) but does not react with squamous cell carcinoma, sarcoma, melanoma or lymphoma. When used in conjunction with MAb to Cytokeratin 1/5/10/14 (human) (34βE12) (Prod. No. ENZ-30903), the majority of epithelium and epithelial tumors are labeled. |
UniProt ID |
P05787 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Long Term Storage |
+4°C |
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Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues: A.M. Gown & A.M. Vogel; Am. J. Pathol. 114, 309 (1984), Abstract — Full Text
- Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies: A.M. Gown & A.M. Vogel; J. Cell Biol. 95, 414 (1982), Abstract — Full Text
Related Products

Alternative Name | HMWCK, High molecular weight cytokeratin, Keratin-903 |
---|---|
Application | IHC (PS), WB |
Host | Mouse |
Isotype | IgG1 |
Species Reactivity | Human |

Alternative Name | CK8 |
---|---|
Application | IHC (PS), WB |
Host | Mouse |
Isotype | IgM |
Species Reactivity | Human |
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
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SDS
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