Inhibitor of actin polymerization
- Highly pure and potent inhibitor of actin polymerization
- Blocker of low conductance potassium channel
- Highly cited
Cytochalasin D is a cell-permeable mycotoxin that binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits. It causes the disruption of actin filaments and inhibition of actin polymerization. As a result, it impedes with a variety of processes including cell cycle, motility, phagocytosis, proliferation, amongst others. It is about 10-fold more effective than cytochalasin B (Prod. No. BML-T108). Cytochalasin D also inactivates low conductance K+ channels, modulates CD4 cross-linking in T lymphocytes, increases intracellular Ca2+ levels, and exhibits antitumor activity.
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Product Details
Alternative Name |
NSC 209835, Zygosporin A |
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Appearance |
White to off-white solid |
CAS |
22144-77-0 |
Couple Target |
Potassium channel |
Couple Type |
Blocker |
Formula |
C30H37NO6 |
MW |
507.6 |
Purity |
≥97% (HPLC, single spot by TLC) |
Solubility |
Soluble in DMSO (25mg/ml), 100% ethanol (5mg/ml), or methylene chloride (10mg/ml). |
Source |
Isolated from Zygosporium mansonii. |
Handling & Storage
Use/Stability |
As indicated on product label or CoA when stored as recommended. |
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Handling |
Protect from light. |
Long Term Storage |
-20°C |
Shipping |
Ambient Temperature |
Regulatory Status |
RUO – Research Use Only |
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- Magnetic colloidal nanoformulations to remotely trigger mechanotransduction for osteogenic differentiation: M. Estévez, et al.; J. Colloid Interface Sci. 15, 664 (2024), Abstract
- Chemical screens for particle-induced macrophage death identifies kinase inhibitors of phagocytosis as targets for toxicity: Tran, U. T., Kitami, T., et al.; J. Nanobiotechnology 22, 621 (2024), Abstract
- Actin cytoskeleton depolymerization increases matrix metalloproteinase gene expression in breast cancer cells by promoting translocation of cysteine-rich protein 2 to the nucleus: Mgrditchian, T., Brown-Clay, J., et al.; Front Cell Dev Biol 11, 1100938 (2023), Abstract
- A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery: Essletzbichler, P., Sedlyarov, V., et al.; Life Sci. Alliance 6, (2023), Abstract
- Dysregulated neutrophil extracellular traps formation in sepsis: M. Mulet, et al.; Clin. Transl. Med. 170, 370 (2023), Abstract
- Facile detection of mechanical forces across proteins in cells with STReTCh: B.L. Zhong, et al.; Cell Rep. Methods 2, 100278 (2022), Abstract
- Melatonin prevents oocyte deterioration due to cotinine exposure in mice: J. Cheng, et al.; Biol. Reprod. 107, 635 (2022), Abstract
- Pointed-end processive elongation of actin filaments by Vibrio effectors VopF and Vop: E. Kudryashova, et al.; Sci. Adv. 8, eadc9239 (2022), Abstract
- Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle: C.H. Oon & K.E. Prehoda; Elife 10, e66574 (2021), Abstract — Full Text
- RhoA drives actin compaction to restrict axon regeneration and astrocyte reactivity after CNS injury: S. Stern, et al.; Neuron 109, 3436 (2021), Abstract
- Actin assembly around the Shigella-containing vacuole promotes successful infection: S. Kuhn, et al.; Cell Rep. 31, 107638 (2020), Abstract — Full Text
- Collective cell sorting requires contractile cortical waves in germline cells: S. Chanet, et al.; Curr. Biol. 30, 4213 (2020), Abstract
- Osmotic gradients induce stable dome morphogenesis on extracellular matrix: S. Ishida-Ishihara, et al.; J. Cell Sci. 133, jcs243865 (2020), Abstract
- A new form of actin assembly around the i>Shigella/i>-containing vacuole regulates its intracellular niche: Kühn, S., Bergqvist, J., et al.; bioRxiv , (2020)
- The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy: Ho, W. Y., Tai, Y. K., et al.; Autophagy 15, 827 (2019), Abstract
- Functional self-assembled neocartilage as part of a biphasic osteochondral construct: W.E. Brown, et al.; PLoS One 13, e0195261 (2018), Abstract — Full Text
- Myosin 2-Induced Mitotic Rounding Enables Columnar Epithelial Cells to Interpret Cortical Spindle Positioning Cues: Chanet, S., Sharan, R., et al.; Curr. Biol. 27, 3350 (2017), Abstract
- The Role of Arp2/3 in Growth Cone Actin Dynamics and Guidance Is Substrate Dependent: J. Miguel-Ruiz, et al.; J. Neurosci. 17, 5895 (2017), Abstract — Full Text
- Merotelic kinetochore attachment in oocyte meiosis II causes sister chromatids segregation errors in aged mice: J. Cheng, et al.; Cell Cycle 15, 140 (2017), Abstract — Full Text
- Porphyromonas gingivalis Outer Membrane Vesicles Induce Selective Tumor Necrosis Factor Tolerance in a Toll-Like Receptor 4- and mTOR-Dependent Manner: Waller, T., Kesper, L., et al.; Infect. Immun. 84, 1194 (2016), Abstract
- The DNA fibers of shrimp hemocyte extracellular traps are essential for the clearance of Escherichia coli: T.H. Ng, et al.; Dev. Comp. Immunol. 48, 229 (2015), Abstract
- IQGAP1 regulates actin cytoskeleton organization in podocytes through interaction with nephrin: Y. Liu, et al.; Cell. Signal. 27, 867 (2015), Application(s): Cell Culture, Abstract
- Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40: S. Hatano, et al.; Int. Immunopharmacol. 25, 321 (2015), Application(s): Cell Culture, Assay, Abstract
- Filopodia-based Wnt transport during vertebrate tissue patterning: E. Stanganello, et al.; Nat. Commun. 6, 5846 (2015), Application(s): Cell Culture, Abstract
- The role of the gap junction protein connexin43 in B lymphocyte motility and migration: S. Machtaler, et al.; FEBS Lett. 588, 1249 (2014), Abstract
- A novel 2.5D culture platform to investigate the role of stiffness gradients on adhesion-independent cell migration: M.P. Pebworth, et al.; PLoS One 9, e110453 (2014), Abstract — Full Text
- Different contributions of clathrin-and caveolae-mediated endocytosis of vascular endothelial cadherin to lipopolysaccharide-induced vascular: Y. Zhang, et al.; PLoS One 9, e106328 (2014), Abstract — Full Text
- Tumor stiffness is unrelated to myosin light chain phosphorylation in cancer cells: Yu, H. J., Serebryannyy, L. A., et al.; PLoS One 8, e79776 (2013), Abstract
- The signalling imprints of nanoparticle uptake by bone marrow derived dendritic cells: L. Karlson Tde, et al.; Methods 60, 275 (2013), Abstract
- Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen: B. Miles, et al.; J. Leukoc. Biol. 2, 281 (2013), Abstract — Full Text
- Histones trigger sterile inflammation by activating the NLRP3 inflammasome: R. Allam, et al.; Eur. J. Immunol. 43, 3336 (2013), Abstract — Full Text
- Transport of influenza virus neuraminidase (NA) to host cell surface is regulated by ARHGAP21 and Cdc42 proteins: S. Wang, et al.; J. Biol. Chem. 287, 9804 (2012), Abstract — Full Text
- Nonmuscle myosin IIB links cytoskeleton to IRE1α signaling during ER stress: He, Y., Beatty, A., et al.; Dev. Cell 23, 1141 (2012), Abstract
- Induction of type I IFN is a physiological immune reaction to apoptotic cell-derived membrane microparticles: Schiller, M., Parcina, M., et al.; J. Immunol. 189, 1747 (2012), Abstract
- Staurosporine and cytochalasin D induce chondrogenesis by regulation of actin dynamics in different way: M. Kim, et al.; Exp. Mol. Med. 44, 521 (2012), Abstract — Full Text
- Evidence for Gardnerella vaginalis uptake and internalization by squamous vaginal epithelial cells: implications for the pathogenesis of bacterial vaginosis: C.N. Marrs, et al.; Microbes Infect. 14, 500 (2012), Abstract — Full Text
- Diabetes-induced increased oxidative stress in cardiomyocytes is sustained by a positive feedback loop involving Rho kinase and PKCβ2.: H. Soliman, et al.; Am. J. Physiol. Heart Circ. Physiol. 303, H989 (2012), Abstract — Full Text
- Thymosin beta4 inhibits TNF-alpha-induced NF-kappaB activation, IL-8 expression, and the sensitizing effects by its partners PINCH-1 and ILK: P. Qiu, et al.; FASEB J. 25, 1815 (2011), Abstract — Full Text
- Interdependency of cell adhesion, force generation and extracellular proteolysis in matrix remodeling: Kirmse, R., Otto, H., et al.; J. Cell Sci. 124, 1857 (2011), Abstract
- Keratinocyte growth factor enhances barrier function without altering claudin expression in primary alveolar epithelial cells: M.J. LaFemina, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 299, L724 (2010), Abstract — Full Text
- Hexokinase translocation during neutrophil activation, chemotaxis, and phagocytosis: disruption by cytochalasin D, dexamethasone, and indomethacin: J.B. Huang, et al.; Cell Immunol. 218, 95 (2002), Abstract
- Signaling pathways involved in thrombin-induced cell protection: F.M. Donovan & D.D. Cunningham; J. Biol. Chem. 273, 12746 (1998), Abstract
- The role of actin-binding protein 280 in integrin-dependent mechanoprotection: M. Glogauer, et al.; J. Biol. Chem. 273, 1689 (1998), Abstract
- Disruption of actin microfilaments by cytochalasin D leads to activation of p53: S. N. Rubtsova, et al.; FEBS Lett. 430, 353 (1998), Abstract
- Myosin-actin interaction plays an important role in human immunodeficiency virus type 1 release from host cells: H. Sasaki, et al.; PNAS 92, 2026 (1995), Abstract
- Cytochalasin D modulates CD4 crosslinking sensitive mitogenic signal in T lymphocytes: A. Aszalos, et al.; Cell. Immunol. 157, 81 (1994), Abstract
- Involvement of actin cytoskeleton in modulation of apical K channel activity in rat collecting duc: W.H. Wang, et al.; Am. J. Physiol. 267, F592 (1994), Abstract
- Effects of cytochalasin and phalloidin on actin: J.A. Cooper; J. Cell. Biol. 105, 1473 (1987), Abstract
- Actin polymerization. The mechanism of action of cytochalasin D: D.W. Goddette & C. Frieden; J. Biol. Chem. 261, 15974 (1986), Abstract
- Action of cytochalasin D on cytoskeletal networks: M. Schliwa; J. Cell. Biol. 92, 79 (1982), Abstract
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Last modified: May 29, 2024
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