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CYTAG^® SuperCGH Labeling Kit

ENZ-GEN120

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Superior labeling efficiency for low input samples, with greater sensitivity and a simpler workflow

Suitable for previously limiting low input samples, including precious biopsy samples
Validated for blood, amniotic fluid, postnatal, and oncology samples
Provides excellent dynamic analytical range down to 50 ng
Shorter workflow has samples ready for hybridization in < 4 hours
Superior DLR scores (0.09-0.12) exceeding industry standards
Ability to perform total genomic DNA analysis without amplification or complexity reduction

Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

With optimized, proprietary reagents, Enzo’s CYTAG^® SuperCGH Labeling Kits for oligo arrays produce high quality data using as little as 50 ng of genomic DNA, without a need for pre-amplification for precious low input samples.

Shipping: Available products typically ship within 24/48h, via priority shipping.

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CYTAG® SuperCGH Labeling Kit Kit box image

CYTAG® SuperCGH Labeling Kit dup_dels — Analysis of patient samples using 50 ng DNA input on oligonucleotide microarray (Agilent 4 x 180K, CGH); A) Patient with Trisomy 9 showing duplication of Chromosome 9 (9p24.2-p11.2): DLRs = 0.149; B) Patient with Jacobsen Syndrome showing deletion of Chromosome 11 (11q24.1-q25): DLRs = 0.123; C) Amniotic sample 3: DLRs = 0.121; D) Amniotic sample 4: DLRs = 0.125

CYTAG® SuperCGH Labeling Kit HighestSpecificActivity — 50 ng DNA samples were labeled with Enzo’s CYTAG^® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor’s kit. Enzo’s proprietary labeling technology generates the highest specific activity of labeling for Cy3 and Cy5.

CYTAG® SuperCGH Labeling Kit SuperiorDyeIncorporation — 50 ng DNA samples were labeled with Enzo’s CYTAG^® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor’s kits.

CYTAG® SuperCGH Labeling Kit LowestDLRScores — Comparative analysis of 50 ng DNA samples labeled with Enzo’s CYTAG^® SuperCGH Labeling Kit or that of a leading competitor demonstrates significantly lower DLR scores.

CYTAG® SuperCGH Labeling Kit DynamicAnalyticalRange — CYTAG^® SuperCGH labels DNA across a dynamic analytical range

CYTAG® SuperCGH Labeling Kit Prenatal_amnios — 50 ng DNA input from 4 different amniotic prenatal samples were labeled using CYTAG^® SuperCGH Labeling Kit and applied to an 8 x 60K array, showing consistently low DLR scores.

Product Details

Application	CGH
Application Notes	Validated on Agilent SurePrint Microarrays.
Contents	-0010 size: ENZ-GEN120-0010: Primers/Reaction Buffer, 1 x 400 µL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 100 µL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 100 µL; Klenow DNA Polymerase, 1 x 40 µL; Stop Buffer, 1 x 100 µL; Nuclease-free Water, 1 x 1 mL; PCR & Gel Clean-up columns, 20 tests -0100 size: ENZ-GEN120-0100: Primers/Reaction Buffer, 1 x 4 mL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 1 mL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 1 mL; Klenow DNA Polymerase, 1 x 0.4 mL; Stop Buffer, 1 x 1 mL; Nuclease-free Water, 1 x 10 mL; PCR & Gel Clean-up columns, 200 tests PCR & Gel Clean-up Columns can be re-ordered using ENZ-GEN100
Technical Info / Product Notes	Application Note: Using CYTAG® SuperCGH Labeling Kit for the Analysis of FFPE Oncological Samples by CGH. Automated Liquid Handling for High Throughput DNA Labeling with CYTAG® SuperCGH Labeling Kit. Sensitivity of Comparative Genomic Hybridization Using Artificial Mosaicism and Small DNA Quantities. Labeling of Small DNA Quantities With CYTAG® SuperCGH Labeling Kit for Prenatal Diagnostics.

Handling & Storage

Use/Stability	Stable for at least one year after receipt when stored as recommended.
Handling	Protect Super Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Systematic in vitro analysis of therapy resistance in glioblastoma cell lines by integration of clonogenic survival data with multi-level molecular data: L.E. Schnöller, et al.; Radiat. Oncol. 18, 51 (2023), Abstract
Comparative analysis of histopathological parameters, genome-wide copy number alterations, and variants in genes involved in cell cycle regulation in chordomas of the skull base and sacrum: H. Salle, et al.; J. Neuropathol. Exp. Neurol. 82, 312 (2023), Application(s): FFPE chordoma DNA, Abstract — Full Text