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CYTAG^® CGH Labeling kit

ENZ-42671

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Superior labeling efficiency and better dye incorporation results in less failed runs

Ability to perform total genomic DNA analysis without amplification or complexity reduction
Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
QC benchmarked and validated using high resolution arrays
Superior DLR scores (0.09-0.12), exceeding industry standards

Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification.

Bulk quantity kit, 2 x 1000 reactions, is available upon special request.

For the purification of labeled DNA, use PCR & Gel Clean-up columns, Prod No. ENZ-GEN100.

Shipping: Available products typically ship within 24/48h, via priority shipping.

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CYTAG® CGH Labeling kit high_spec_activity

CYTAG® CGH Labeling kit image — Analysis of syndromic DNA using an oligonucleotide microarray (Agilent 4 x 180K) demonstrated the characteristic deletion in 15q11.2-q13 (chromosome 15) found in patients with Prader-Willi syndrome.

Product Details

Application	CGH
Application Notes	Validated on Agilent SurePrint, OGT CytoSure™, and Illumina 24Sure Microarrays.
Contents	-K010 size: Primers/Reaction Buffer, 1 x 400 µL; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µL; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µL; Klenow DNA Polymerase, 1 x 20 µL; Stop Buffer, 1 x 100 µL; Nuclease-free Water, 1 x 1 mL -K100 size: Primers/Reaction Buffer, 1 x 4 mL; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 mL; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 mL; Klenow DNA Polymerase, 1 x 0.2 mL; Stop Buffer, 1 x 1 mL; Nuclease-free Water, 1 x 10 mL
Technical Info / Product Notes	Application Notes: Compatibility of CYTAG® CGH Labeling Kit from Enzo Life Sciences with SurePrint® G3 Human CGH 1x1M Microarrays from Agilent Compatibility of CYTAG® CGH Labeling Kit with Illumina 24sure® Microarrays CGH labeling protocol for small volumes of DNA sample High Resolution Microarray Scanner used to Compare CGH Labeling Methods Cited samples: For an overview of Cited Samples by Sample Type, please click here. For an overview of Cited Samples by Array Type, please click here.

Handling & Storage

Use/Stability	Stable until expiration date when stored as recommended.
Handling	Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Assessing BRCA1 activity in DNA damage repair using human induced pluripotent stem cells as an approach to assist classification of BRCA1 variants of uncertain significance: M. Ozgencil, et al.; PLoS One 16, e0260852 (2021), Abstract
Composite neuroblastoma metastatic to a lymph node: the novel histopathologic diagnosis of a unique multiclonal neoplasm: J.L. Kurtz, et al.; Ann. Clin. Lab. Sci. 51, 573 (2021), Abstract
Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing: A. Mishra, et al.; Carcinogenesis. 41, 334 (2020), Abstract — Full Text
Non-classical 1p36 deletion in a patient with Duane retraction syndrome: case report and literature review: E. Yokoyama, et al.; Case Reports 13, 42 (2020), Abstract — Full Text
Further support linking the 22q11.2 microduplication to an increased risk of bladder exstrophy and highlighting LZTR1 as a candidate gene: J. Lundin, et al.; Mol. Genet. Genomic. Med. 7, e666 (2019), Abstract — Full Text
Polymer simulations of heteromorphic chromatin predict the 3D folding of complex genomic loci: A. Buckle, et al.; Mol. Cell. 72, 786 (2018), Abstract — Full Text
Derivative chromosomes involving 5p large rearranged segments went unnoticed with the use of conventional cytogenetics: E. Yokoyama, et al.; Mol. Cytogenet. 11, 30 (2018), Abstract — Full Text
Evaluation of the ISL1 gene in the pathogenesis of bladder exstrophy in a Swedish cohort: S. Arkani, et al.; Hum. Genome Var. 5, 18009 (2018), Abstract — Full Text
Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin: A. Petrova, et al.; Stem. Cells. Dev. 25, 1366 (2016), Application(s): Induced pluripotent stem cell lines, Abstract
Comparison of clinical grade human platelet lysates for cultivation of mesenchymal stromal cells from bone marrow and adipose tissue: M. Juhl, et al.; Scand. J. Clin. Lab. Invest. 76, 93 (2016), Application(s): Labeling of DNA from bone marrow mesenchymal stem cells and adipose tissue-derived stromal cells, Abstract

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CYTAG^® CGH Labeling kit

ENZ-42671

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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CYTAG® CGH Labeling kit

ENZ-42671

Product Details

Handling & Storage

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