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Cysteinyl Leukotriene ELISA kit

ADI-901-070

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Highly sensitive ELISA kit enabling detection of LTC₄/D₄/E₄ in 4 hours.

Sensitive measurement of LTC₄/D₄/E₄, detecting as little as 26.6 pg/ml
High throughput format with results in 4 hours for up to 38 samples in duplicate
Fully quantitative results that surpass semi-quantitative Western blot analysis
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The Cysteinyl Leukotriene ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. Safe non-radioactive measurement of LTC₄/D₄/E₄. Ready-to-use liquid color-coded reagents reduce errors.

Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B₄ (LTB₄) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB₄ has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC₄, LTD₄, and LTE₄) are formed by addition of cysteine derivatives to LTA₄. The conversion of LTA₄ to LTC₄ by the enzyme LTC₄ synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

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Product Details

Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of Cysteinyl Leukotrienes (LTC₄/D₄/E₄) in culture supernatants and urine from any species.
Assay Time	4 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxM Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Sensitivity	26.6 pg/ml (range 78.1 – 2,500 pg/ml)
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Use/Stability	Store all components at +4°C, except standard and conjugate at -20°C.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Inhibition of adenovirus transport from the endosome to the cell nucleus by rotenone: M.B. Manzanero, et al.; Front. Pharmacol. 14, 1293296 (2024), Abstract
Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes: A. Gainullina, et al.; Cell Rep. 42, 112046 (2023), Abstract
Highly potent and selective 5-lipoxygenase inhibition by new, simple heteroaryl-substituted catechols for treatment of inflammation: V. Krauth, et al.; Biochem. Pharmacol. 208, 115385 (2023), Abstract
Platelets control liver tumor growth through P2Y12-dependent CD40L release in NAFLD: C. Ma, et al.; Cancer Cell 40, 986 (2022), Abstract
Cysteinyl leukotrienes and acetylcholine are biliary tuft cell cotransmitters: M. Keshavarz, et al.; Sci. Immunol. 7, eabf6734 (2022), Abstract
Cysteinyl leukotriene induces eosinophil extracellular trap formation via cysteinyl leukotriene 1 receptor in a murine model of asthma: A.A. da Cunha, et al.; Exp. Lung Res. 1080, 1902148 (2021), Abstract
Attenuating effect of Peruvian cocoa populations on the acute asthmatic response in Brown Norway rats: M. Periz, et al.; Nutrients 12, 2301 (2020), Application(s): Analysis of rat BALF, Abstract — Full Text
Development and characterization of an allergic asthma rat model for interventional studies: M. Periz, et al.; Int. J. Mol. Sci. 21, 3841 (2020), Application(s): Analysis of rat BALF, Abstract — Full Text
Gliotoxin from Aspergillus fumigatus Abrogates Leukotriene B4 Formation through Inhibition of Leukotriene A4 Hydrolase: König, S., Pace, S., et al.; Cell Chem. Biol. 26, 524 (2019), Abstract
Immunomodulatory and inhibitory effect of Immulina®, and Immunloges® in the Ig-E mediated activation of RBL-2H3 cells. A new role in allergic inflammatory: K. Appel, et al.; Plants 7, 13 (2018), Application(s): ELISA using culture supernatants, Abstract — Full Text

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