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Protein synthesis inhibitor
Antibiotic. Inhibits protein synthesis in eukaryotes by interfering with the translocation step. Induces apoptosis in tumor cell lines but can also delay or inhibit apoptosis induced by other agents. Widely used for selection of resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression. At 10µg/ml cycloheximide totally inhibits protein synthesis in mouse thymocytes.
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Product Details
| Activity |
Potency is >900units/mg. |
|---|---|
| Alternative Name |
Actidione, Naramycin A, 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide |
| Appearance |
White to light yellow powder. |
| CAS |
66-81-9 |
| Formula |
C15H23NO4 |
| MI |
14: 2728 |
| MW |
281.3 |
| Purity |
≥90% (HPLC) |
| RTECS |
MA4375000 |
| Solubility |
Soluble in acetone, chloroform, ethanol, and methanol. Nearly insoluble in water. |
| Source |
Isolated from Streptomyces griseus. |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. |
|---|---|
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature – Dangerous Good |
| Regulatory Status |
RUO – Research Use Only |
|---|
- A GluN2B disease-associated variant promotes degradation of NMDA receptors via autophagy: Benske, T. M., Williams, M. P., et al.; bioRxiv , (2025)
- Proinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2: T. Cremer, et al.; PLoS One 19, e0287877 (2024), Abstract — Full Text
- N4BP1 functions as a dimerization-dependent linear ubiquitin reader which regulates TNF signalling: K.W. Kliza, et al.; Cell Death Discov. , (2024), Abstract
- The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells: Galiger, C., Zohora, F. T., et al.; BMC Cancer 23, 1148 (2023), Abstract
- Cigarette smoking is a secondary cause of folliculin loss: Li, X., Lai, Y., et al.; Thorax 78, 402 (2023), Abstract
- piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer: S. Wang, et al.; Cell Death Dis. 13, 465 (2022), Abstract
- The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors: Whittsette, A. L., Wang, Y. J., et al.; iScience 25, 104754 (2022), Abstract
- Quantitative interactome proteomics identifies proteostasis network for GABAsub>A/sub> receptors: Wang, Y., Di, X., et al.; bioRxiv , (2022)
- Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors: Wang, Y. J., Di, X. J., et al.; J. Biol. Chem. 298, 102423 (2022), Abstract
- Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci. 13, 48 (2022), Abstract
- The Dual Histone Deacetylase-Proteasome Inhibitor RTS-V5 Acts Synergistically With Ritonavir to Induce Endoplasmic Reticulum Stress in Bladder Cancer Cells: K. Okubo, et al.; Anticancer Res. 41, 5987 (2021), Abstract
- Proteostasis Regulators Restore Function of Epilepsy-Associated GABAA Receptors: Di, X. J., Wang, Y. J., et al.; Cell Chem. Biol. 28, 46 (2021), Abstract
- A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance: Liu, Y., Jurczak, M. J., et al.; Nat. Chem. Biol. 17, 298 (2021), Abstract
- Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia: Lai, Y., Li, X., et al.; Cell Death Dis. 12, 828 (2021), Abstract
- The Cdc48 Complex Alleviates the Cytotoxicity of Misfolded Proteins by Regulating Ubiquitin Homeostasis: Higgins, R., Kabbaj, M. H., et al.; Cell Rep. 32, 107898 (2020), Abstract
- Cigarette smoke extract modulates Pseudomonas aeruginosa bacterial load via USP25/HDAC11 axis in lung epithelial cells: Long, C., Lai, Y., et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 318, L252 (2020), Abstract
- L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams: VanDussen, K. L., Sonnek, N. M., et al.; Stem Cell Res. 37, 101430 (2019), Abstract
- Atg14 protects the intestinal epithelium from TNF-triggered villus atrophy: Jung, H., Leal-Ekman, J. S., et al.; Autophagy 15, 1990 (2019), Abstract
- Inverse Regulation of Lipocalin-2/24p3 Receptor/SLC22A17 and Lipocalin-2 Expression by Tonicity, NFAT5/TonEBP and Arginine Vasopressin in Mouse Cortical Collecting Duct Cells mCCD(cl.1): Implications for Osmotolerance: Probst, S., Scharner, B., et al.; Int. J. Mol. Sci. 20, (2019), Abstract
- Autophagy is a gatekeeper of hepatic differentiation and carcinogenesis by controlling the degradation of Yap: Y.A. Lee, et al.; Nat. Commun. 9, 4962 (2018), Abstract — Full Text
- LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells: Long, C., Lai, Y., et al.; Biochim. Biophys. Acta Gene Regul. Mech. 1861, 773 (2018), Abstract
- Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence: Miller, I., Min, M., et al.; Cell Rep. 24, 1105 (2018), Abstract
- Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCFFbxl17-recognized acetyldegron: Lai, Y., Li, J., et al.; J. Cell Sci. 130, 3578 (2017), Abstract
- An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response: A. De Gassart, et al.; PNAS 113, E117 (2016), Application(s): Cell culture, Abstract — Full Text
- Bombyx E75 isoforms display stage- and tissue-specific responses to 20-hydroxyecdysone: K. Li, et al.; Sci. Rep. 5, 12114 (2015), Application(s): Cell Culture, Abstract — Full Text
- The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737: Boiani, M., Daniel, C., et al.; J. Biol. Chem. 288, 6980 (2013), Abstract
- Molecular mechanisms of bortezomib resistant adenocarcinoma cells: Suzuki, E., Demo, S., et al.; PLoS One 6, e27996 (2012), Abstract
- Specific dual effect of cycloheximide on B lymphocyte apoptosis: involvement of CPP32/caspase-3: Cl Lemaire et al.; Biochem. Pharmacol. 58, 85 (1999), Abstract
- Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties: C. Christner, et al.; J. Med. Chem. 42, 3615 (1999), Abstract
- Calpain inhibitors and serine protease inhibitors can produce apoptosis in HL-60 cells: Q. Lu & R.L. Mellgren; Arch. Biochem. Biophys. 334, 175 (1996), Abstract
- A novel protein expressed in mammalian cells undergoing apoptosis: R.J. Grand et al.; Exp. Cell. Res. 218, 439 (1995), Abstract
- Mercuric chloride-induced programmed cell death of a murine T cell hybridoma. I. Effect of the proto-oncogene Bcl-2: J. Alten et al.; Cell. Immunol. 161, 98 (1995), Abstract
- Regulation of bcl-2, bcl-XL and bax in the control of apoptosis by hematopoietic cytokines and dexamethasone: J. Lotem & L. Sachs; Cell. Growth. Differ. 6, 647 (1995), Abstract
- Reevaluation of the role of de novo protein synthesis in rat thymocyte apoptosis: S.C. Chow, et al.; Exp. Cell Res. 216, 149 (1995), Abstract
- DNA fragmentation induced in macrophages by gliotoxin does not require protein synthesis and is preceded by raised inositol triphosphate levels: P. Waring; J. Biol. Chem. 265, 14476 (1990), Abstract — Full Text
- Early estrogen-induced metabolic changes and their inhibition by actinomycin D and cycloheximide in human breast cancer cells: 31P and 13C NMR studies: M. Neeman & H. Degani; PNAS 86, 5585 (1989), Abstract — Full Text
- Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation in rat fibroblasts: R.B. Widelitz, et al.; Mol. Cell. Biol. 6, 1088 (1986), Abstract — Full Text
- The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes: T.G. Obrig, et al.; J. Biol. Chem. 246, 174 (1971), Abstract — Full Text
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