CuAAC Reaction Ligand Test Kit (THPTA & BTTAA based)

The CuAAC Reaction Ligand Test Kit is suitable to perform Copper (Cu(I))-catalyzed Azide-Alkyne Click chemistry reactions (CuAAC) with Azide- or Alkyne- modified biomolecules and cells containing metabolically functionalized Alkyne- or Azide-modified biomolecules. It allows a direct comparison of BTTAA and THPTA to find the most suitable Cu(I)-stabilizing ligand for your experimental set-up. 1 Kit provides sufficient amounts to perform 25 CuAAC experiments each with THPTA and BTTAA à 200 μl using 2 mM CuSO4 (copper source), 10 mM THPTA or 10 mM BTTAA (Cu(I)-stabilizing ligands) and 100 mM Na-Ascorbate (reduction reagent) in 100 mM Na-Phosphate reaction buffer.