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Cpn10 polyclonal antibody

ADI-SPA-110

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Chaperonin 10 (Cpn10 or Hsp10), is the ~10 kDa mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human Cpn10 is identical with Early pregnancy factor (EPF), which is involved in control over cell growth and development.

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This antibody is covered by our Worry-Free Guarantee.

Cpn10 polyclonal antibody Immunohistochemistry — IHC Analysis: Human testis tissue stained with Cpn10, pAb at 10µg/ml.

Cpn10 polyclonal antibody Western blot — Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Cpn10 (Hsp10) pAb.

Product Details

Alternative Name	HSP10, HSPE1, GroES, Heat shock protein 10, Chaperonin 10
Application	IHC (PS), WB
Application Notes	Detects a band of ~10kDa by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	NM_002157 (RefSeq)
Host	Rabbit
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of human Cpn10.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:2,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Dog, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Technical Info / Product Notes	Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000115541).
UniProt ID	P61604
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Mitochondrial alterations in fibroblasts from sporadic Alzheimer’s disease (AD) patients correlate with AD-related clinical hallmarks: Eysert, F., Kinoshita, P. F., et al.; Acta Neuropathol. Commun. 12, 90 (2024), Abstract
Mitochondria alterations in fibroblasts from sporadic Alzheimer’s disease (AD) patients correlate with AD-related clinical hallmarks: Eysert, F., Kinoshita, P., et al.; bioRxiv , (2023), Reactant(s): Human
Impairment of mitochondrial unfolded protein response contribute to resistance declination of H2 O2 -induced injury in senescent MRC-5 cell model: T.Y. Lee, et al.; Kaohsiung J. Med. Sci. 36, 89 (2020), Abstract — Full Text
Effect of Tim23 knockdown in vivo on mitochondrial protein import and retrograde signaling to the UPRmt in muscle: Oliveira, A. N., Hood, D. A., et al.; Am. J. Physiol. Cell Physiol. 315, C516 (2018), Abstract
The unfolded protein response in relation to mitochondrial biogenesis in skeletal muscle cells: Mesbah Moosavi, Z. S., Hood, D. A., et al.; Am. J. Physiol. Cell Physiol. 312, C583 (2017), Abstract
Chronology of UPR activation in skeletal muscle adaptations to chronic contractile activity.: Memme, J. M., Oliveira, A. N., et al.; Am. J. Physiol. Cell Physiol. 310, C1024 (2016), Application(s): WB / Reactant(s): Rat, Abstract
Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats: Cheng, Y., Sun, J., et al.; Exp. Ther. Med. 12, 2759 (2016), Abstract
Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone: S. Corvera, et al.; Mol. Cell. Biol. 23, 1085 (2003), Application(s): WB using mouse samples, Abstract
Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation: B. Schumacher, et al.; Ann. Thorac. Surg. 74, 767 (2002), Application(s): WB using human samples, Abstract
Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells: S. Orrenius, et al.; EMBO J. 18, 2040 (1999), Application(s): WB using human samples, Abstract

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Cpn10 polyclonal antibody

ADI-SPA-110

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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