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CPH . hydrochloride

ALX-430-078

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Spin probe reagent

Effective, cell permeable and non-toxic spin probe for the detection of superoxide radical and peroxynitrite, both in vitro and in vivo. Resistant to reduction by vitamin C and thiols.

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Product Details

Alternative Name	1-Hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine . HCl
Appearance	White to off-white solid.
CAS	164364-89-0
Formula	C₉H₁₇NO₃ . HCl
MW	223.7
Purity	≥99% (EPR)
Solubility	Soluble in DMSO, 100% ethanol, methanol or water.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Prepare fresh solutions daily before use and store 4-6 hours on ice under a nitrogen/argon flow.Oxygen will lead to oxidation and therefore it is strongly recommended to dissolve the probe in water and not in organic solvents such as DMSO, ethanol o
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Imaging Reactive Oxygen Radicals in Excised Mouse Lung Trapped by Reaction with Hydroxylamine Probes Using 1 GHz Rapid Scan Electron Paramagnetic Resonance: H.B. Elajaili, et al.; Mol. Imaging Biol. 26, 503 (2023), Abstract
Sex differences in blood pro-oxidant status and platelet activation in children admitted with respiratory syncytial virus bronchiolitis: a pilot study: I.T. De Jacobis, et al.; Ital. J. Pediatr. 46, 29 (2020), Abstract — Full Text
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K: H.B. Eajaili, et al.; J. Vis. Exp. , e58461 (2019), Abstract
An altered redox balance and increased genetic instability characterize primary fibroblasts derived from Xeroderma pigmentosum group A patients: E. Parlanti, et al.; Mutat. Res. 782, 34 (2015), Application(s): Measurement of ROS levels, Abstract
EPR Detection of Cellular and Mitochondrial Superoxide Using Cyclic Hydroxylamines: S.I. Dikalov, et al.; Free. Radic. Res. 45, 417 (2011), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract — Full Text
Therapeutic targeting of mitochondrial superoxide in hypertension: A.E. Dikalova, et al.; Circ. Res. 107, 106 (2010), Abstract
Indoxyl sulfate induces complex redox alterations in mesangial cells: A.K. Gelasco & J.R. Raymond; Am. J. Physiol. Renal Physiol. 290, F1551 (2006), Abstract
Interactions of peroxynitrite with uric acid in the presence of ascorbate and thiols: implications for uncoupling endothelial nitric oxide synthase: N. Kuzkaya, et al.; Biochem. Pharmacol. 70, 343 (2005), Abstract
Interactions of peroxynitrite, tetrahydrobiopterin, ascorbic acid, and thiols: implications for uncoupling endothelial nitric-oxide synthase: N. Kuzkaya, et al.; J. Biol. Chem. 278, 22546 (2003), Abstract — Full Text
Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine: S.I. Dikalov, et al.; Arch. Biochem. Biophys. 402, 218 (2002), Abstract

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Spin probe reagent

28 citations

Alternative Name	1-Hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine . HCl
Purity	≥99% (EPR)

Last modified: August 15, 2025

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CPH . hydrochloride

ALX-430-078

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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SDS

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