Home Shop Collagen I, rat tail

Collagen I, rat tail

ALX-522-435

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Main structural protein in the extracellular matrix

New formulation enhances gelling over a larger concentration range.

Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.

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Collagen I, rat tail SDS-PAGE — Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, rat tail.

Collagen I, rat tail CHO — Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.

Product Details

Activity	Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips.
Alternative Name	COL1
Appearance	Hazy viscous liquid.
Application Notes	Can be used for preparation of collagen gels, thin layer coating of surfaces (culture plates, slides, coverslips).
Concentration	5mg/ml
Endotoxin Content	<100 EU/mg purified protein (LAL test)
Formulation	Sterile liquid. In 0.02N acetic acid.
MW	235kDa, 215kDa, 130kDa, 115kDa
Purity	≥90% (SDS-PAGE)
Source	Isolated from rat tail tendons.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Keep sterile. Do not freeze.
Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Glypican 3 as target therapy to prevent cell migration and proliferation in rhabdomyosarcoma: Bacchiega, M., D’Agostino, S., et al.; Sci. Rep. 15, 20913 (2025), Abstract
Glypican 3 as target therapy to prevent cell migration and proliferation in rhabdomyosarcoma: Bacchiega, M., D’Agostino, S., et al.; Research Square , (2025)
A human model to deconvolve genotype-phenotype causations in lung squamous cell carcinoma: Ogden, J., Sellers, R., et al.; Nat. Commun. 16, 3215 (2025), Abstract
Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroids: Chen, J., Hnath, B., et al.; PNAS 121, e2405717121 (2024), Abstract
Five near-infrared-emissive graphene quantum dots for multiplex bioimaging: Valimukhametova, A., Fannon, O., et al.; 2d Mater. 11, (2024), Abstract
Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts: F.R. Augello, et al.; Biomedicines 11, 2410 (2023), Abstract
Doped Graphene Quantum Dots as Biocompatible Radical Scavenging Agents: Bhaloo, A., Nguyen, S., et al.; Antioxidants (Basel) 12, (2023), Abstract
Modeling Gas Plasma-Tissue Interactions in 3D Collagen-Based Hydrogel Cancer Cell Cultures: L. Miebach, et al.; Bioengineering (Basel) 10, 367 (2023), Abstract
A novel human model to deconvolve cell-intrinsic phenotypes of genetically dysregulated pathways in lung squamous cell carcinoma: Ogden, J., Sellers, R., et al.; bioRxiv , (2023)
Dia1 coordinates differentiation and cell sorting in a stratified epithelium: Harmon, R. M., Devany, J., et al.; J. Cell Biol. 221, (2022), Abstract

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

Firm Gelling Procedure
(Note: The ideal concentration for gelling is 1 - 5mg/ml. The end user will need to determine the appropriate concentration for their specific purpose).

Ammonium Hydroxide Gas Method

Dilute 5mg/ml collagen to the desired concentration using sterile conditions. (Note: Lower concentrations will have reduced rigidity).
Add enough collagen to cover the surface.
Saturate a cotton ball or piece of filter paper with concentrated ammonium hydroxide and make a closed vapor chamber.
Heat to 37°C until gel forms.
Remove the ammonium hydroxide.
Soak the gel surface in PBS for 1 hour.
Wash several times PBS.
Store the gel in PBS at +4°C until use.
Equilibrate the gel in the desired media for 30 minutes prior to use.

NaOH Method (For 10ml)

Sterilely add 1- 7ml of 5mg/ml collagen to a tube depending on desired final concentration. (Note: Lower concentrations will have reduced rigidity).
Add 1ml of sterile 10X PBS.
Add 1 ml of sterile 1N NaOH.
Bring final volume to 10ml with sterile dH₂O
Cover the desired surface with a layer of the collagen and place at 37°C until the gel has solidified. Gel will only form if the pH is ≥7.0.
Store in PBS at +4°C until use.
Equilibrate the gel in the desired media for 30 minutes prior to use.

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