Main structural protein in the extracellular matrix
- New formulation enhances gelling over a larger concentration range.
Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.
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Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.


Product Details
Activity |
Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips. |
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Alternative Name |
COL1 |
Appearance |
Hazy viscous liquid. |
Application Notes |
Can be used for preparation of collagen gels, thin layer coating of surfaces (culture plates, slides, coverslips). |
Concentration |
5mg/ml |
Endotoxin Content |
<100 EU/mg purified protein (LAL test) |
Formulation |
Sterile liquid. In 0.02N acetic acid. |
MW |
235kDa, 215kDa, 130kDa, 115kDa |
Purity |
≥90% (SDS-PAGE) |
Source |
Isolated from rat tail tendons. |
Handling & Storage
Use/Stability |
As indicated on product label or CoA when stored as recommended. |
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Handling |
Keep sterile. Do not freeze. |
Short Term Storage |
+4°C |
Long Term Storage |
+4°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Glypican 3 as target therapy to prevent cell migration and proliferation in rhabdomyosarcoma: Bacchiega, M., D’Agostino, S., et al.; Sci. Rep. 15, 20913 (2025), Abstract
- Glypican 3 as target therapy to prevent cell migration and proliferation in rhabdomyosarcoma: Bacchiega, M., D’Agostino, S., et al.; Research Square , (2025)
- A human model to deconvolve genotype-phenotype causations in lung squamous cell carcinoma: Ogden, J., Sellers, R., et al.; Nat. Commun. 16, 3215 (2025), Abstract
- Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroids: Chen, J., Hnath, B., et al.; PNAS 121, e2405717121 (2024), Abstract
- Five near-infrared-emissive graphene quantum dots for multiplex bioimaging: Valimukhametova, A., Fannon, O., et al.; 2d Mater. 11, (2024), Abstract
- Doped Graphene Quantum Dots as Biocompatible Radical Scavenging Agents: Bhaloo, A., Nguyen, S., et al.; Antioxidants (Basel) 12, (2023), Abstract
- A novel human model to deconvolve cell-intrinsic phenotypes of genetically dysregulated pathways in lung squamous cell carcinoma: Ogden, J., Sellers, R., et al.; bioRxiv , (2023)
- Modeling Gas Plasma-Tissue Interactions in 3D Collagen-Based Hydrogel Cancer Cell Cultures: L. Miebach, et al.; Bioengineering (Basel) 10, 367 (2023), Abstract
- Dia1 coordinates differentiation and cell sorting in a stratified epithelium: Harmon, R. M., Devany, J., et al.; J. Cell Biol. 221, (2022), Abstract
- Dual-Mode Fluorescence/Ultrasound Imaging with Biocompatible Metal-Doped Graphene Quantum Dots: Valimukhametova, A. R., Zub, O. S., et al.; ACS Biomater. Sci. Eng. 8, 4965 (2022), Abstract
- Differential gene expression response of synovial fibroblasts from temporomandibular joints and knee joints to dynamic tensile stress: Nazet, U., Neubert, P., et al.; J. Orofac. Orthop. 83, 361 (2022), Abstract
- A TNF receptor 2 agonist ameliorates neuropathology and improves cognition in an Alzheimer’s disease mouse model: N.O. Casan, et al.; PNAS 119, e2201137119 (2022), Abstract
- Efficacy of probiotic Streptococcus thermophilus in counteracting TGF-β1-induced fibrotic response in normal human dermal fibroblasts: F. Lombardi, et al.; J. Inflamm. 19, 27 (2022), Abstract
- Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis: N.M. Ghahhari, et al.; Nat. Commun. 13, 2104 (2022), Abstract
- SIRT1-mediated deacetylation of FOXO3a transcription factor supports pro-angiogenic activity of interferon-deficient tumor-associated neutrophils: S. Bordbari, et al.; Int. J. Cancer 150, 1198 (2022), Abstract
- Axon guidance receptor ROBO3 modulates subtype identity and prognosis via AXL-associated inflammatory network in pancreatic cancer: N. Krebs, et al.; JCI Insight 7, e154475 (2022), Abstract
- Low nanogel stiffness favors nanogel transcytosis across an in vitro blood-brain barrier: L. Ribovski, et al.; Nanomedicine 34, 102377 (2021), Abstract
- Generation and Encapsulation of Human iPSC-Derived Vascular Smooth Muscle Cells for Proangiogenic Therapy: Dash, B. C., Hsia, H. C., et al.; Methods Mol. Biol. 2549, 259 (2021), Abstract
- Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint: Nazet, U., Feulner, L., et al.; Cells 10, (2021), Abstract
- Integrin β3 targeting biomaterial preferentially promotes secretion of bFGF and viability of iPSC-derived vascular smooth muscle cells: B. C. Dash, et al.; Biomater. Sci. 9, 5319 (2021), Abstract
- A new perfusion culture method with a self-organized capillary network: K. Sugihara, et al.; PLoS One 15, e0240552 (2020), Abstract — Full Text
- Induced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing: J. Gorecka, et al.; Regen. Med. 15, 1277 (2020), Abstract — Full Text
- Increased Lamin B1 Levels Promote Cell Migration by Altering Perinuclear Actin Organization: Fracchia, A., Asraf, T., et al.; Cells 9, (2020), Abstract
- The anti-inflammatory peptide Catestatin blocks chemotaxis: Muntjewerff, E. M., Parv, K., et al.; bioRxiv , (2020)
- A filter-free blood-brain barrier model to quantitatively study transendothelial delivery of nanoparticles by fluorescence spectroscopy: E. De Jong, et al.; J. Control. Release 289, 14 (2018), Abstract
- Effects of selexipag and its active metabolite in contrasting the profibrotic myofibroblast activity in cultured scleroderma skin fibroblasts: M. Cutolo, et al.; Arthritis Res. Ther. 20, 77 (2018), Abstract — Full Text
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Specific Protocol
Firm Gelling Procedure
(Note: The ideal concentration for gelling is 1 - 5mg/ml. The end user will need to determine the appropriate concentration for their specific purpose).
Ammonium Hydroxide Gas Method
- Dilute 5mg/ml collagen to the desired concentration using sterile conditions. (Note: Lower concentrations will have reduced rigidity).
- Add enough collagen to cover the surface.
- Saturate a cotton ball or piece of filter paper with concentrated ammonium hydroxide and make a closed vapor chamber.
- Heat to 37°C until gel forms.
- Remove the ammonium hydroxide.
- Soak the gel surface in PBS for 1 hour.
- Wash several times PBS.
- Store the gel in PBS at +4°C until use.
- Equilibrate the gel in the desired media for 30 minutes prior to use.
NaOH Method (For 10ml)
- Sterilely add 1- 7ml of 5mg/ml collagen to a tube depending on desired final concentration. (Note: Lower concentrations will have reduced rigidity).
- Add 1ml of sterile 10X PBS.
- Add 1 ml of sterile 1N NaOH.
- Bring final volume to 10ml with sterile dH2O
- Cover the desired surface with a layer of the collagen and place at 37°C until the gel has solidified. Gel will only form if the pH is ≥7.0.
- Store in PBS at +4°C until use.
- Equilibrate the gel in the desired media for 30 minutes prior to use.
Certificate of Analysis
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SDS
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