Coelenterazine (ultra pure)

Colenterazine is useful for monitoring calcium in live cells and tissues, in reporter gene assays, for superoxide anion detection and in drug high-throughput screening applications. Coelenterazine is useful for reconstituting aequorin in cells that have been transfected with apo-aequorin cDNA. The aequorin complex comprises a 22kDa apoaequorin protein, molecular oxygen and coelenterazine. In the presence of Ca(II), coelenterazine is oxidized to coelenteramide, yielding the release of carbon dioxide and blue light. Unlike fluorescent Ca(II) indicators, Ca²⁺-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Wavelength Maxima: Excitation 429nm, Emission 466nm