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cGMP complete ELISA kit

ADI-901-164

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Most sensitive (0.043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.

Versatile and convenient kit to assay both intracellular and extracellular cGMP
High sensitivity with an optional acetylation protocol which increases sensitivity 10-fold (0.059 pmol/ml)
Reliable and consistent lot-to-lot performance
Higher throughput format with results in just 3 hours for up to 39 samples in duplicate
Simple, efficient and well-established sample handling protocols
Save with bulk kit package!

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.

Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.

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Product Details

Alternative Name	cyclic GMP
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR Microtiter plate, Conjugate, Antibody, Assay buffer 2 concentrate, 0.1 M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
Crossreactivity	cyclic GMP (100%) and <0.001%: GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP
Sensitivity	Assay Buffer : 0.42 pmol/ml (non-acetylated) 0.043 pmol/ml (acetylated) HCl: 0.604 pmol/ml (non-acetylated) 0.059 pmol/ml (acetylated) (range 0.8 – 500 pmol/ml (non-acetylated); 0.08 – 50 pmol/ml (acetylated))
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Use/Stability	Store all components at +4°C, except standard and conjugate at -20°C.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Pharmacological Stimulation of Soluble Guanylate Cyclase Counteracts the Profibrotic Activation of Human Conjunctival Fibroblasts: Fioretto, B. S., Rosa, I., et al.; Cells 13, (2024), Abstract
Association of natriuretic peptides and receptor activity with cardio-metabolic health at middle age: Prickett, T. C. R., Espiner, E. A., et al.; Sci. Rep. 14, 9919 (2024), Abstract
Dysregulated Cyclic Nucleotide Metabolism in Alcohol-Associated Steatohepatitis: Implications for Novel Targeted Therapies: D.M. Durango, et al.; Biology 12, 1321 (2023), Abstract
Protein-based vaccine candidate MecVax broadly protects against enterotoxigenic Escherichia coli intestinal colonization in a rabbit model: I. Upadhyay, et al.; Infect. Immun. 91, e0027223 (2023), Abstract
Hypoxic erythrocytes mediate cardioprotection through activation of soluble guanylate cyclase and release of cyclic GMP: Yang, J., Sundqvist, M. L., et al.; J. Clin. Invest. 133, (2023), Abstract
Effects of a Soluble Guanylate Cyclase Stimulator Riociguat on Contractility of Isolated Pulmonary Artery and Hemodynamics of U46619-Induced Pulmonary Hypertension in Dogs: S. Kameshima, et al.; Vet. Sci. 10, 159 (2023), Abstract
Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE-/- mice fed a high fat diet: Liu, Y., Croft, K. D., et al.; Eur. J. Nutr. 62, 1845 (2023), Abstract
Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin: E.D. Zegeye, et al.; Vaccines 10, 241 (2022), Abstract
Broadening the Spectrum of Loss-of-Function Variants in NPR-C-Related Extreme Tall Stature: P. Lauffer, et al.; J. Endocr. Soc. 6, bvac019 (2022), Abstract
Selective PDE1 inhibition ameliorates vascular function, reduces inflammatory response, and lowers blood pressure in ageing animals: K. Golshiri, et al.; J. Pharmacol. Exp. Ther. 378, 173 (2022), Abstract

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