CFSE

ALX-610-030

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Amine dye

Non-toxic cell permeable fluorescent dye that reacts with amine compounds. Readily accumulates inside viable cells. Hardly leaks from cells when covalently attached to intracellular proteins. Hydrolyzed CFSE emits fluorescence (Ex(max): 495nm; Em(max): 519nm). Therefore, CFSE is utilized for the long-term observation of cell activities by fluorescent microscopy as well as for viable cell detection.

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Product Details

Alternative Name	5(6)-(N-Succinimidyloxycarbonyl)-3′,6′,0,0′-diacetylfluorescein, 5(6)-Carboxyfluorescein diacetate, N-succinimidyl ester-
Appearance	White or slightly yellow powder.
CAS	150347-59-4
Formula	C₂₉H₁₉NO₁₁
MW	557.5
Purity	≥95% (HPLC)
Solubility	Soluble in DMSO, dimethyl formamide or methanol.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The Endothelial Glycocalyx and Retinal Hemodynamics: G. Kaur, et al.; Pathophysiology 29, 663 (2022), Abstract
Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question: E.J. Suchin, et al.; J. Immunol. 166, 973 (2001), Abstract
Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes: S.W. Kamau, et al.; BMC Pharmacol. 1, 1 (2001), Abstract
Flow cytometric cell division tracking using nuclei: J. Hasbold & P.D. Hodgkin; Cytometry 40, 230 (2000), Abstract
Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro: H.E. Gruber, et al.; Biotech. Histochem. 75, 118 (2000), Abstract
Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution: A.B. Lyons; J. Immunol. Methods 243, 147 (2000), Abstract
Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven: B.F. de St Groth, et al.; Immunol. Cell Biol. 77, 530 (1999), Abstract
The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo: J. Mintern, et al.; Immunol. Cell Biol. 77, 539 (1999), Abstract
Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester: A.B. Lyons; Immunol. Cell Biol. 77, 509 (1998), Abstract
A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy: S. Oehen, et al.; J Immunol Methods 207, 33 (1997), Abstract

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CFSE

ALX-610-030

Product Details

Handling & Storage

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