Simple, fast and accurate assay to measure cell proliferation and cytotoxicity
- One-step, ready-to-use solution with no radioisotopes
- High sensitivity that correlates with the [3H]-thymidine incorporation assay
- High-throughput screening without a solubilization step
- More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.
It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
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Product Details
Alternative Name |
CCKi-8 |
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Application |
Colorimetric detection |
Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
Contents |
WST-8 solution, 1-methoxy-PMS |
Quantity |
100 tests: 1 mL |
Technical Info / Product Notes |
Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium. |
Handling & Storage
Handling |
Protect from light. Avoid freeze/thaw cycles. |
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Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Therapeutic Effect of Melittin-dKLA Targeting Tumor-Associated Macrophages in Melanoma: I.H. Han, et al.; Int. J. Mol. Sci. 23, 3094 (2022), Abstract
- Human constitutive androstane receptor represses liver cancer development and hepatoma cell proliferation by inhibiting erythropoietin signaling: Z. Li, et al.; J. Biol. Chem. 298, 101885 (2022), Abstract
- lncRNA AGAP2-AS1 Facilitates Tumorigenesis and Ferroptosis Resistance through SLC7A11 by IGF2BP2 Pathway in Melanoma: L. An, et al.; Comput. Math. Methods Med. 2022, 1972516 (2022), Abstract
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- Overexpression of SIRT3 Suppresses Oxidative Stress-induced Neurotoxicity and Mitochondrial Dysfunction in Dopaminergic Neuronal Cells: S. Lee, et al.; Exp. Neurobiol. 30, 341 (2021), Abstract
- Vitamin B12 Reduces TDP-43 Toxicity by Alleviating Oxidative Stress and Mitochondrial Dysfunction: Y.M. Jeon, et al.; Antioxidants 11, 82 (2021), Abstract
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- Genetic and Functional Analysis of Glycosyltransferase 8 Domain–Containing Protein 1 in Taiwanese Patients With Amyotrophic Lateral Sclerosis: P.C. Tsai, et al.; Neurol. Genet. 7, e267 (2021), Abstract
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- Synthesis, properties and in vitro photodynamic activity of water-soluble azaphthalocyanines and azanaphthalocyanines: P. Zimcik, et al.; Photochem. Photobiol. 86, 168 (2010), Abstract
- Markers of tumor-initiating cells predict chemoresistance in breast cancer: C. Gong, et al.; PLoS One 5, e15630 (2010), Abstract — Full Text
- Circulating Toll-like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C: J.P. Wang, et al.; APMIS 118, 261 (2010), Abstract
- Interleukin-1beta increases gap junctional communication among synovial fibroblasts via the extracellular-signal-regulated kinase pathway: C. Niger, et al.; Biol. Cell 102, 37 (2009), Abstract — Full Text
- COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells: H. Radilova, et al.; Mol. Cell Biochem. 330, 131 (2009), Abstract
- Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect: Y. Kim, et al.; J. Biol. Chem. 280, 23273 (2005), Abstract — Full Text
- Cyclin G2 dysregulation in human oral cancer: Y. Kim, et al.; Cancer Res. 64, 8980 (2004), Abstract — Full Text
- Mitogen-activated protein kinases mediate peroxynitrite-induced cell death in human bronchial epithelial cells: E. Nabeyrat, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L1112 (2003), Abstract — Full Text
- Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000), Abstract
- Enhancement of MTT, a tetrazolium salt, exocytosis by amyloid beta- protein and chloroquine in cultured rat astrocytes: I. Isobe, et al.; Neurosci. Lett. 266, 129 (1999), Abstract
- A water-soluble tetrazolium salt useful for colorimetric cell viability assay: H. Tominaga, et al.; Anal. Commun. 36, 47 (1999)
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- A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet: M. Ishiyama, et al.; Biol. Pharm. Bull. 19, 1518 (1996), Abstract
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