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CEACAM1 (CD66a, BGP) belongs to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAMs.
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Immunohistological staining of colon tissue with MAb to CEACAM1 (GM8G5) (Prod. No. ALX-805-076). Cryosections of colon tissue were stained with 10 µg/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representa-tive sections of analysed tissues. Detection of GM8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate. Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.
Specificity testing of GM8G5. BOSC cells were transiently transfected with an expression vector containing the cDNA of CEACAM1. Expression of the constructs was tested with MAb to CEACAM1 (GM8G5) (Prod. No. ALX-805-076) (B, purple curves); an irrelevant monoclonal antibody served as negative control (red curve). For specificity testing (A), GM8G5 hybridoma supernatant was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM1 transfected cells (purple curve).
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RUO – Research Use Only |
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