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Immunofluorescence staining of CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDKN2A polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.
CDKN2A was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg 293T Whole Cell Lysate 2 µL anti-CDKN2A rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G. Primary antibody: Anti-CDKN2A rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Observed band size: 17 kDa.
Anti-CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Observed band size:17 kDa.
| Regulatory Status |
RUO – Research Use Only |
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