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Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is implicated in the development and/or progression of selected cancer types.
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Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ENZ-ABS722 at 2-5µg for 1×106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
ICC/IF analysis of CD46 in HeLa cells. The cell was stained with ENZ-ABS722 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
The cell lysates of Ramos, HeLa and A549 (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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RUO – Research Use Only |
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