Home Shop CD45 (human) monoclonal antibody (MEM-28)

CD45 (human) monoclonal antibody (MEM-28)

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This antibody is covered by our Worry-Free Guarantee.

Application	Flow Cytometry, ICC, IHC (PS), IP, WB
Clone	MEM-28
Formulation	Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
Host	Mouse
Immunogen	Human thymocytes and T lymphocytes.
Isotype	IgG1
Purity Detail	Protein A-affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (1µg/ml)Immunocytochemistry (10µg/ml, paraformaldehyde fixation can be used)Immunohistochemistry (paraffin sections, no pre-treatment of tissue sections is essential)Western Blot (1µg/ml; Positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS.Application note: non-reducing conditions. SDS-PAGE (6% separating gel))Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human
Specificity	Recognizes all isoforms of CD45.
UniProt ID	P08575
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Regulatory Status	RUO – Research Use Only

PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer: D.J. Wozniak, et al.; Nat. Commun. 8, 1508 (2017), Abstract — Full Text
Characterization of endothelial-like cells derived from human mesenchymal stem cells: Liu, J. W., Dunoyer-Geindre, S., et al.; J. Thromb. Haemost. 5, 826 (2007), Abstract
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules: L. Cermak, et al.; J. Biol. Chem. 277, 7955 (2002), Abstract — Full Text
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74): V. Horejsi, et al.; Folia Biol (Praha) 34, 23 (1988), Abstract
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen: V. Horejsi, et al.; Folia Biol (Praha) 32, 12 (1986), Abstract

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