CD40L, soluble (mouse) (recombinant) set

CD154, TNFSF 5, gp39

ELISA, FUNC

Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034).

CD40L: 0.1mg/ml after reconstitution.Enhancer: 1mg/ml after reconstitution.

10µg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2×50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1’000-fold.

<0.1EU/µg purified protein (LAL test; Bio Whittaker).

Lyophilized. Contains PBS.

≥90% (SDS-PAGE).

Reconstitute CD40L with 100µl sterile water and each vial of enhancer with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

CD40L binds to human and mouse CD40 in an ELISA assay.

CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.
FLAG is a registered trademark of Sigma-Aldrich Co.

P27548 (CD40L)