CD40L (soluble) (mouse), (recombinant)

CD154, TNFSF 5, gp39

ELISA: binds to human CD40 at 10-500 ng/ml.

0.1mg/ml after reconstitution.

<50EU/mg purified protein (LAL test; Associates of Cape Cod).

Lyophilized. Contains PBS.

~26kDa (SDS-PAGE).

≥90% (SDS-PAGE)

Purified by multi-step chromatography.

Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

Binds to human and mouse CD40.

Historical data has shown that mouse CD40L stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer (see Set Prod. No. ALX-850-075).

FLAG is a registered trademark of Sigma-Aldrich Co.

P27548