CD40L, soluble (human) (recombinant) set

CD154, TNFSF 5

FUNC

Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046).

CD40L: 0.1mg/ml after reconstitutionEnhancer: 1mg/ml

10µg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2×50µg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold.

CD40L: Lyophilized. Contains PBS.
Enhancer: Liquid. In PBS, pH 7.4, containing 50% glycerol. The formulation contains no antimicrobial preservatives.

≥95% (SDS-PAGE).

Reconstitute CD40L with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.

CD40L binds to human CD40.

CD40L (Prod. No. ALX-522-015) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.

P29965