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Schematic structure of human CD40L (peptide, aa. 116 to 261).
SDS-PAGE Analysis of CD40L (soluble) (human), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg.
![2µg/ml of goat anti-human µ chain antibody and with the indicated concentration of CD40L in the presence and absence of 1µg/ml enhancer (Prod. No. ALX-804-034). Cells were pulsed for an additional 6 hours with [3H]thymidine (1µCi/well) and harvested. [3H]thymidine incorporation was monitored by liquid scintillation counting."""""](https://www.enzo.com/wp-content/uploads/2023/01/522-015-2.gif)
Figure 2: Stimulation of B cells by CD40L, Soluble (human) (rec.). Dose dependent costimulation of CD19+ cells by CD40L in the presence of 10ng/ml of IL-4 and of 2µg/ml anti-µ antibodies. Note that in the presence of enhancer (Prod. No. ALX-804-034)
![2µg/ml of goat anti-human µ chain antibody and with the indicated concentration of CD40L in the presence and absence of 1µg/ml enhancer (Prod. No. ALX-804-034). Cells were pulsed for an additional 6 hours with [3H]thymidine (1µCi/well) and harvested. [3H]thymidine incorporation was monitored by liquid scintillation counting."""""](https://www.enzo.com/wp-content/uploads/2023/01/522-015-2.gif?w=516)
| Regulatory Status |
RUO – Research Use Only |
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Related Products
| Alternative Name | CD154, TNFSF 5, gp39 |
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| Purity | ≥90% (SDS-PAGE) |
| Source | Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
MEGACD40L® Protein (soluble) (human), (recombinant)
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High activity, high purity CD40L protein for co-stimulatory activation of an immune response
| Alternative Name | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless |
|---|---|
| Purity | ≥90% (SDS-PAGE) |
| Source | Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
MEGACD40L® Protein (soluble) (mouse), (recombinant)
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High activity, high purity CD40L protein for co-stimulatory activation of an immune response
| Alternative Name | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF5:ACRP30headless, gp39:ACRP30headless |
|---|---|
| Purity | ≥95% (SDS-PAGE) |
| Source | Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
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| Purity | ≥95% (SDS-PAGE). |
|---|