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CD40 (human):Fc (human), (recombinant)

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Alternative Name	TNFRSF 5
Application Notes	ELISA: binds to CD40L at 1-10 ng/ml. Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression.
Concentration	1mg/ml after reconstitution.
Endotoxin Content	<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc).
Formulation	Lyophilized. Contains PBS.
MW	~54kDa (SDS-PAGE).
Purity	≥95% (SDS-PAGE)
Reconstitution	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Source	Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.
Specificity	Binds human and mouse CD40L (CD154).
UniProt ID	P25942

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

How CD40L reverse signaling regulates axon and dendrite growth: Carriba, P., Davies, A. M., et al.; Cell. Mol. Life Sci. 78, 1065 (2021), Abstract
Signalling Pathways Mediating the Effects of CD40-Activated CD40L Reverse Signalling on Inhibitory Medium Spiny Neuron Neurite Growth: Carriba, P., Davies, A. M., et al.; Cells 10, (2021), Abstract
CD40L Reverse Signaling Influences Dendrite Spine Morphology and Expression of PSD-95 and Rho Small GTPases: Carriba, P., Wyatt, S., et al.; Front Cell Dev Biol 8, 254 (2020), Abstract
CD40-targeting KGYY15 peptides do not efficiently block the CD40-CD40L interaction: Bojadzic, D., Buchwald, P., et al.; Diabetologia 62, 2158 (2019), Abstract
CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons: P. Carriba & A.M. Davies; Elife 6, e30442 (2017), Abstract — Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000), Abstract

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Negative control for Fc chimeric proteins

Alternative Name	Thy-1 (RLE mutant)
Purity	≥90% (SDS-PAGE)
Source	Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins.

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