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CD18 (human) monoclonal antibody (MEM-148)

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Alternative Name	β2 integrin
Application	Flow Cytometry, FUNC, IP, WB
Application Notes	Functional Application: induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.
Clone	MEM-148
Crossreactivity	Does not cross-react with pig CD18.
Formulation	Liquid. In PBS, pH 7.4, containing 15 mM sodium azide.
Host	Mouse
Immunogen	Human peripheral blood mononuclear cells.
Isotype	IgG1
Purity Detail	Protein A-affinity purified.
Recommendation Dilutions/Conditions	Western Blot (non-reducing)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human
Specificity	Recognizes free CD18 (β2 integrin) not associated with CD11.
UniProt ID	P05107
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells: K. Drbal, et al.; Blood 98, 1561 (2001), Abstract — Full Text
A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins: K. Drbal, et al.; Immunobiology 203, 687 (2001), Abstract
Human leukocytes contain a large pool of free forms of CD18: K. Drbal, et al.; BBRC 275, 295 (2000), Abstract

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