CD137 (human):Fc (human), (recombinant)

4-1BB, TNFRSF 9

ELISA: binds to CD137L at 10-100 ng/ml.
Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD137.
Functional Application: Induces proliferation of monocytes.
Note: Induction of monocyte proliferation by CD137 required immobilization of the CD137 protein, e.g. by coating of the protein to the surface of the tissue culture dishes.

1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).

Lyophilized. Contains PBS.

~50kDa (SDS-PAGE).

≥95% (SDS-PAGE)

Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in HEK 293 cells. The cysteine-rich region of human CD137 (4-1BB) (aa 1-186) is fused to the Fc portion of human IgG1.

Binds human CD137L (4-1BBL).

Q07011