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CD134L (soluble) (human), (recombinant)

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Alternative Name	OX40L, TNFSF 4, CD252
Application Notes	ELISA: binds to CD134 receptor). Can be used together with anti-FLAG^® MAb to detect membrane-bound human CD134 (OX40).
Concentration	0.1mg/ml after reconstitution.
Endotoxin Content	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
Formulation	Lyophilized. Contains PBS.
MW	~30kDa (SDS-PAGE).
Purity Detail	Purified by single-step affinity chromatography.
Reconstitution	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Source	Produced in HEK 293 cells. The extracellular domain of human CD134L (OX40L) (aa 52-183) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
Specificity	Binds to human CD134 (OX40).
Technical Info / Product Notes	Historical data indicates that CD134L activates T cell proliferation and delays neutrophil apoptosis. FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	P23510

Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

A novel method for determination of the affinity of protein: protein interactions in homogeneous assays: P. Newton, et al.; J. Biomol. Screen. 13, 674 (2008), Abstract
Enhancing the immunostimulatory function of dendritic cells by transfection with mRNA encoding OX40 ligand: J. Dannull, et al.; Blood 105, 3206 (2005), Abstract
Functional expression of CD134 by neutrophils: R. Baumann, et al.; Eur. J. Immunol. 34, 2268 (2004), Abstract

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