Home Shop CCR8 (human) (NT) polyclonal antibody

CCR8 (human) (NT) polyclonal antibody

ALX-210-762

5 citations

Datasheet
Manual
SDS
CofA

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Product Details

Alternative Name	C-C chemokine receptor 8, CD198
Application	ELISA, Flow Cytometry, IHC, WB
Formulation	Affinity purified antibody in 10mM KHPO4, 140mM NaCl, 1mg/ml BSA and 0.1% sodium azide.
Host	Goat
Immunogen	Synthetic peptide corresponding to aa 12-36 (V12TDYYYPDIFSSPCDAELIQTNGKL36) of the N-terminal extracellular domain of human CCR8 (C-C chemokine receptor 8).
Purity Detail	Epitope-affinity purified IgG.
Recommendation Dilutions/Conditions	ELISA (1:50,000)Flow Cytometry (2-6µl per 106 cells/50-100µl)Immunohistochemistry (1:125)Western blot (1:500)Optimal conditions must be determined individually for each application.
Species Reactivity	Human
UniProt ID	P51685
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

CCL16 activates an angiogenic program in vascular endothelial cells: M. Strasly, et al.; Blood 103, 40 (2004), Abstract
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque; Blood 97, 39 (2001), Abstract
CC Chemokine I-309 is the Principal Monocyte Chemottractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque; Circulation 102, 786 (2000), Abstract
The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem. 273, 386 (1998), Abstract — Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol. 26, 3021 (1996), Abstract

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

Immunohistochemistry on paraffin sections:
- Fix paraffin sections in 10% formalin.
- De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 min. at room temperature (RT).
- Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block non-specific binding sites.
- Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 min. at RT.
- Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 min.
- Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 min.
- Wash slides and incubate with DAB for 5-7 min. at RT.
- Wash slides with PBS and counter stain with hematoxylin.

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