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Cathepsin S drug discovery kit

BML-AK431

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A QUANTIZYME^® Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.

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Cathepsin S drug discovery kit Fig1a — Example of inhibitor data. Using the control, the slope is equal to 34.28 RFU/sec. With the inhibitor (E-64), the slope is equal to 0 RFU/sec. The % activity remaining after using the inhibitor can be calculated as follow: (0/34.28) x 100 = 0%

Cathepsin S drug discovery kit Fig2 — Kinetics of Z-VVR-AMC cleavage by Cathepsin S, 0.059mU/well; 37°C. Rates were obtained from the slope of the initial, linear portion of plots of AFU vs. time. Curve and kinetic parameters derived from a non-linear least squares fit to the Michaelis-Menten equation (Marquadt algorithm).

Product Details

Application	Activity assay, Fluorescent detection, HTS
Application Notes	Complete system designed to screen modulators of cathepsin S activity. The assays are performed in a convenient 96-well microplate format.
Contents	Cathepsin S enzyme (human) (recombinant), AMC substrate, AMC calibration standard, inhibitor, assay buffer, 1/2-volume white microplate.
UniProt ID	P25774

Handling & Storage

Use/Stability	The enzyme component should be thawed as close to the time of use as is possible, and held on ice while in use. The enzyme can be frozen and thawed up to 5 times, but care should be taken to minimize both the time the enzyme spends on ice, and the number of times it is frozen and thawed. Do not hold the diluted enzyme at assay temperature (e.g 37°C) for an extended time before starting the assay.
Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation: A.T. Reddy, et al.; PLoS One 11, e0153336 (2016), Application(s): Cat S enzyme inhibition assay, Abstract — Full Text

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Cathepsin S drug discovery kit

BML-AK431

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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