Catalase Activity Assay

Hydrogen peroxide is a common metabolic byproduct of many cellular processes. A potent oxidant, it’s toxic due to its ability to damage cell structures, proteins and DNA. Catalase evolved to remove hydrogen peroxide and is one of the most efficient enzymes with turnover numbers approaching 3×106 s -1. It acts in maintaining a level of ROS in cells necessary for normal physiological functions without allowing harm from excessive amounts. AkrivisBio’s Catalase Activity Assay is a simple, sensitive way to measure catalase activity in a range of biological samples. In the assay, any hydrogen peroxide not consumed by catalase is utilized by peroxidase to convert ADHP to resorufin with strong absorbance and fluorescence.