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Figure:
(A) Schematic structure of caspase-8 and its cleavage products as detected by anti-caspase-8 (12F5). The antibody is directed against the p18 subunit and detects procaspase-8 (p55/54), the intermediate cleavage products of 43kD and 41kD and the p18 active subunit of caspase-8.
(B) Detection of Fas ligand-induced caspase-8 processing and activation in human Jurkat cells.
Method: Jurkat cells were treated with Fas ligand (100ng/ml). After the indicated time points cell lysates were prepared and separated on a 12.5% SDS-PAGE (2×106 cells/lane) under reducing conditions. Proteins were immunoblotted with anti-caspase-8 12F5 (1µg/ml). Following incubation with peroxidase-conjugated secondary antibodies caspase-8 processing was detected by enhanced chemoluminescent staining. The open arrowhead indicates the two isoforms of procaspase-8 (caspase-8/a and caspase-8/b), which are cleaved into the intermediate forms p43 and p41 and finally processed to the active p18 subunit (filled arrowheads).
Product Details
| Alternative Name |
FLICE |
|---|---|
| Application |
IHC (FS), IP, WB |
| Clone |
12F5 |
| Crossreactivity |
Does not cross-react with mouse caspase-8. |
| Formulation |
Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA and 0.01% thimerosal. |
| Host |
Mouse |
| Immunogen |
Recombinant human caspase-8. |
| Isotype |
IgG2b |
| Purity Detail |
Protein G-affinity purified. |
| Recommendation Dilutions/Conditions |
Western Blot (1:100)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity |
Human |
| Specificity |
Recognizes procaspase-8 and active caspase-8. Detects procaspase-8 (p55/54), the intermediate cleavage products (p43/41) and the p18 active subunit of caspase-8 by Western blot. |
| UniProt ID |
Q14790 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. |
|---|---|
| Long Term Storage |
+4°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicity: Roets, L. E., Blayney, J. K., et al.; Cell Death Dis. 16, 476 (2025), Abstract
- The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor: Otani, K., Komatsu, R., et al.; Int. J. Mol. Sci. 26, 2237 (2025), Abstract
- NLRC5 senses NAD+ depletion, forming a PANoptosome and driving PANoptosis and inflammation: Sundaram, B., Pandian, N., et al.; Cell 187, 4061 (2024), Abstract
- The E3 ubiquitin ligase Itch regulates death receptor and cholesterol trafficking to affect TRAIL-mediated apoptosis: Holloway, J., Seeley, A., et al.; Cell Death Dis. 15, 40 (2024), Application(s): WB, Abstract
- Trauma-associated extracellular histones mediate inflammation via a MYD88-IRAK1-ERK signaling axis and induce lytic cell death in human adipocytes: Roos, J., Zinngrebe, J., et al.; Cell Death Dis. 15, 285 (2024), Abstract
- Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency: Oda, H., Manthiram, K., et al.; Nat. Immunol. 25, 764 (2024), Abstract
- Classical apoptotic stimulus, staurosporine, induces lytic inflammatory cell death, PANoptosis: Sarkar, R., Choudhury, S. M., et al.; J. Biol. Chem. 300, 107676 (2024), Abstract
- A comparative study of apoptosis, pyroptosis, necroptosis, and PANoptosis components in mouse and human cells: Choudhury, S. M., Sarkar, R., et al.; PLoS One 19, e0299577 (2024), Abstract
- Anthrax lethal toxin and tumor necrosis factor-α synergize on intestinal epithelia to induce mouse death: X. Gao, et al.; Protein Cell 15, 135 (2024), Abstract
- Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharide: Cristaldi, M., Buscetta, M., et al.; Cell Death Dis. 14, 773 (2023), Abstract
- The autophagic protein FYCO1 controls TNFRSF10/TRAIL receptor induced apoptosis and is inactivated by CASP8 (caspase 8).: Coppola, V., Marino, I., et al.; Autophagy 19, 2733 (2023), Reactant(s): Human, Abstract
- Anti-Proliferative Effect of Radiotherapy and Implication of Immunotherapy in Anaplastic Thyroid Cancer Cells.: Wächter, S., Roth, S., et al.; Life (Basel) 13, (2023), Application(s): WB, Abstract
- NLRP12-PANoptosome activates PANoptosis and pathology in response to heme and PAMPs.: Sundaram, B., Pandian, N., et al.; Cell 186, 2783 (2023), Application(s): IP / Reactant(s): Human, Abstract
- The E3 ubiquitin ligase NEDD4 regulates chemoresistance to 5-fluorouracil in colorectal cancer cells by altering JNK signalling: Anand, S., Nedeva, C., et al.; Cell Death Dis. 14, 828 (2023), Abstract
- Evaluation of Caspase Activation to Assess Innate Immune Cell Death: Han, J. H., Tweedell, R. E., et al.; J. Vis. Exp. , (2023), Abstract
- The non-apoptotic function of Caspase-8 in negatively regulating the CDK9-mediated Ser2 phosphorylation of RNA polymerase II in cervical cancer: R. Mandal, et al.; Cell. Mol. Life Sci. 79, 597 (2022), Abstract
- Human LUBAC deficiency leads to autoinflammation and immunodeficiency by dysregulation in TNF-mediated cell death: Oda, H., Manthiram, K., et al.; medRxiv , (2022), Reactant(s): Human
- Pancancer transcriptomic profiling identifies key PANoptosis markers as therapeutic targets for oncology.: Mall, R., Bynigeri, R. R., et al.; NAR Cancer 4, zcac033 (2022), Application(s): WB / Reactant(s): Human, Abstract
- ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection.: Karki, R., Lee, S., et al.; Sci. Immunol. 7, eabo6294 (2022), Application(s): WB, Abstract
- Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells: Planès, R., Pinilla, M., et al.; Mol. Cell 82, 2385 (2022), Abstract
- Anti-Tumoral Effect of Chemerin on Ovarian Cancer Cell Lines Mediated by Activation of Interferon Alpha Response.: Schmitt, M., Gallistl, J., et al.; Cancers (Basel) 14, (2022), Application(s): WB / Reactant(s): Human, Abstract
- Proteasome inhibition triggers the formation of TRAIL receptor 2 platforms for caspase-8 activation that accumulate in the cytosol: C.T. Hellwig, et al.; Cell Death Differ. 29, 147 (2022), Abstract
- Direct cleavage of caspase-8 by herpes simplex virus 1 tegument protein US11: M.M. Pizzo, et al.; Sci. Rep. 12, 12317 (2022), Abstract
- Inflammatory Cell Death, PANoptosis, Mediated by Cytokines in Diverse Cancer Lineages Inhibits Tumor Growth: R.K.S. Malireddi, et al.; Immunohorizons 5, 568 (2021), Application(s): WB, Abstract
- TAK1-inhibitors are cytotoxic for multiple myeloma cells alone and in combination with melphalan.: Starheim, K. K., Hella, H., et al.; Oncotarget 12, 2158 (2021), Application(s): WB / Reactant(s): Human, Abstract
- Smac-mimetics reduce numbers and viability of human osteoclasts.: Starheim, K. K., Nonstad, U., et al.; Cell Death Discov. 7, 36 (2021), Application(s): WB, Abstract
- Exploring the MEN1 dependent modulation of caspase 8 and caspase 3 in human pancreatic and murine embryo fibroblast cells: N. Wagener, et al.; Apoptosis , (2021), Abstract
- Clinical Positioning of the IAP Antagonist Tolinapant (ASTX660) in Colorectal Cancer: Crawford, N., Stott, K. J., et al.; Mol. Cancer Ther. 20, 1627 (2021), Abstract
- Selective HSP90β inhibition results in TNF and TRAIL mediated HIF1α degradation: Heck, A. L., Mishra, S., et al.; Immunobiology 226, 152070 (2021), Abstract
- Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation: Douglas, T., Saleh, M., et al.; J. Biol. Chem. 295, 5216 (2020), Abstract
- A revised model of TRAIL-R2 DISC assembly explains how FLIP(L) can inhibit or promote apoptosis: Humphreys, L. M., Fox, J. P., et al.; EMBO Rep. 21, e49254 (2020), Abstract
- USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination: J. Roedig, et al.; EMBO Rep. 2020, e50163 (2020), Application(s): IP, Abstract
- The Covalent CDK7 Inhibitor THZ1 Enhances Temsirolimus-Induced Cytotoxicity via Autophagy Suppression in Human Renal Cell Carcinoma: P.M. Chow, et al.; Cancer Lett. 471, 27 (2020), Abstract
- Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.: Saleh, M., Douglas, T., et al.; Cell Rep. 29, 3652 (2019), Reactant(s): Human, Abstract
- Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages: M. Buscetta, et al.; FASEB J. 34, 1819 (2019), Abstract — Full Text
- Hsp60 and IL-8 axis promotes apoptosis resistance in cancer.: Kumar, S., O’Malley, J., et al.; Br. J. Cancer 121, 934 (2019), Reactant(s): Human, Abstract
- BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity: A.M. Heijink, et al.; Nat. Commun. 10, 100 (2019), Application(s): WB, Abstract — Full Text
- Epigenetically Enhanced PDT Induces Significantly Higher Levels of Multiple Extrinsic Pathway Apoptotic Factors than Standard PDT, Resulting in Greater Extrinsic and Overall Apoptosis of Cutaneous T-cell Lymphoma: Salva, K. A., Kim, Y. H., et al.; Photochem. Photobiol. 94, 1058 (2018), Abstract
- BAX/BAK-Induced Apoptosis Results in Caspase-8-Dependent IL-1β Maturation in Macrophages: D. Chauhan, et al.; Cell Rep. 25, 2354 (2018), Abstract
- Sensitization of glioblastoma cells to TRAIL-induced apoptosis by IAP- and Bcl-2 antagonism.: Lincoln, F. A., Imig, D., et al.; Cell Death Dis. 9, 1112 (2018), Application(s): IP, WB, Abstract
- Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations: S.C. Lee, et al.; Cancer Cell 34, 225 (2018), Abstract
- The poly(ADP-ribose) polymerase inhibitor olaparib induces up-regulation of death receptors in primary acute myeloid leukemia blasts by NF-κB activation: I. Faraoni, et al.; Cancer Lett. 423, 127 (2018), Reactant(s) Human, Abstract
- PU.1 supports TRAIL-induced cell death by inhibiting NF-κB-mediated cell survival and inducing DR5 expression.: Torbett, B. E., Frese, S., et al.; Cell Death Differ. 24, 866 (2017), Reactant(s): Human, Abstract
- The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3: Gaidt, M. M., Ebert, T. S., et al.; Cell 171, 1110 (2017), Abstract
- hvTRA, a novel TRAIL receptor agonist, induces apoptosis and sustained growth retardation in melanoma.: Flørenes, V. A., Prasmickaite, L., et al.; Cell Death Discov. 2, 16081 (2016), Application(s): WB / Reactant(s): Mouse, Abstract
- Regulation of RIPK3- and RHIM-dependent Necroptosis by the Proteasome: Moriwaki, K., Chan, F. K., et al.; J. Biol. Chem. 291, 5948 (2016), Abstract
- Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia: Schirmer, M., Trentin, L., et al.; Cell Death Dis. 7, e2052 (2016), Abstract
- Myxoma Virus Induces Ligand Independent Extrinsic Apoptosis in Human Myeloma Cells: Bartee, M. Y., Dunlap, K. M., et al.; Clin. Lymphoma Myeloma Leuk. 16, 203 (2016), Abstract
- Predicting the cell death responsiveness and sensitization of glioma cells to TRAIL and temozolomide.: Murphy, B. M., Rehm, M., et al.; Oncotarget 7, 61295 (2016), Application(s): WB, Abstract
- Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis. 6, e1800 (2015), Application(s): Immunoprecipitation, Abstract — Full Text
- Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes: E. Kaemmerer, et al.; World J Gastroenterol 21, 4499 (2015), Application(s): Western Blot, Abstract — Full Text
- Multiple mechanisms mediate resistance to sorafenib in urothelial cancer.: Schulz, W. A., Schmitz, I., et al.; Int. J. Mol. Sci. 15, 20500 (2014), Reactant(s): Human, Abstract
- TRAIL-receptor costimulation inhibits proximal TCR signaling and suppresses human T cell activation and proliferation: Lehnert, C., Weiswange, M., et al.; J. Immunol. 193, 4021 (2014), Abstract
- Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes: S.A. Nagel, et al.; Mol. Cell Endocrinol. 382, 368 (2014), Application(s): WB using human cell lysates, Abstract
- Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer: J.S. Riley, et al.; Cell Death Dis. 4, e951 (2013), Application(s): WB using human cell lysates, Abstract — Full Text
- Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent: J.L. Hurwitz, et al.; Eur. J. Cancer 48, 1096 (2012), Application(s): WB using human cell and tissue lysates, Abstract
- Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis: S. Conus, et al.; J. Biol. Chem. 287, 21142 (2012), Application(s): WB using human cell lysates, Abstract — Full Text
- A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): WB using human neutrophil lysates, Abstract — Full Text
- GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation: K. Moriwaki, et al.; J. Biol. Chem. 286, 43123 (2011), Application(s): WB using human cell lysates, Abstract — Full Text
- Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma: B. Gillissen, et al.; J. Cell Biol. 188, 851 (2010), Abstract
- Caspase 8 and menin expressions are not correlated in human parathyroid tumors.: Yu, D., Wada, S., et al.; Endocr. J. 57, 825 (2010), Reactant(s): Human, Abstract
- The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract
- The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Application(s): WB using human follicular lymphoma lysate, Abstract — Full Text
- Antigen activation and impaired Fas-induced death-inducing signaling complex formation in T-large-granular lymphocyte leukemia: Yang, J., Epling-Burnette, P. K., et al.; Blood 111, 1610 (2008), Abstract
- A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem. 283, 13174 (2008), Abstract
- Trypanosoma cruzi posttranscriptionally up-regulates and exploits cellular FLIP for inhibition of death-inducing signal: Hashimoto, M., Nakajima-Shimada, J., et al.; Mol. Biol. Cell 16, 3521 (2005), Abstract
- Addition of 5-fluorouracil to doxorubicin-paclitaxel sequence increases caspase-dependent apoptosis in breast cancer cell lines: Zoli, W., Ulivi, P., et al.; Breast Cancer Res. 7, R681 (2005), Abstract
- Constitutive caspase activation and impaired death-inducing signaling complex formation in CD95-resistant, long-term activated, antigen-specific T cells: G. Strauss, et al.; J. Immunol. 171, 1172 (2003), Abstract — Full Text
- Role of Smac in human leukaemic cell apoptosis and proliferation.: Jia, L., Newland, A. C., et al.; Oncogene 22, 1589 (2003), Application(s): WB / Reactant(s): Human, Abstract
- An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis: Y. Kim, et al.; J. Biol. Chem. 277, 22320 (2002), Abstract — Full Text
- Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001), Abstract — Full Text
- Trail-induced apoptosis in Type I leukemic cells is not enhanced by overexpression of bax: L. Jia, et al.; BBRC 283, 1037 (2001), Abstract
- Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem. 275, 24357 (2000), Abstract — Full Text
- IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract
- P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death: D. Ferrari, et al.; FEBS Lett. 447, 71 (1999), Abstract
- Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999), Abstract — Full Text
- Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32): M. Bitzer, et al.; J. Virol. 73, 702 (1999), Abstract — Full Text
- Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998), Abstract — Full Text
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