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This antibody is covered by our Worry-Free Guarantee.

Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with Caspase-3, mAb (31A1067) at 4µg/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.

Figure 1: Western blot analysis for detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3, which generates 17 and 12kDa, larger and smaller catalytic subunit, respectively.

Figure 3: Western blot analysis of Caspase-3 in A) RAW and B) NIH 3T3 using MAb to Caspase-3 (31A1067) (Prod. No. ALX-804-305) 2µg/ml.

Figure 4: Immunohistochemical analysis of caspase-3 in formalin-fixed, paraffin-embedded human stomach tissue using using MAb to Caspase-3 (31A1067) (Prod. No. ALX-804-305) at 5 ug/ml. High (top panel) and low magnificaton (bottom panel). Caspase-3 staining is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active caspase 3. In most cell types and model systems, cells with active caspase-3 are undergoing apoptosis.





Product Details
Application |
IHC (FS), IHC (PS), WB |
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Clone |
31A1067 |
Formulation |
Liquid. In PBS with 0.05% sodium azide. |
Host |
Mouse |
Immunogen |
Recombinant human caspase-3. |
Isotype |
IgG1 |
Purity Detail |
Protein G-affinity purified. |
Recommendation Dilutions/Conditions |
Western Blot (2-5µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Species Reactivity |
Human, Mouse, Rat |
Specificity |
Recognizes procaspase-3 and cleaved 17kDa catalytic subunit of caspase-3. Recognizes an epitope in the large (17kDa) domain subunit of caspase-3. |
UniProt ID |
P42574 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Use/Stability |
Stable for 6 months when stored at +4°C. |
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Handling |
Avoid freeze/thaw cycles. |
Short Term Storage |
+4°C |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Spatial sequestration of activated-caspase 3 in aggresomes mediates resistance of neuroblastoma cell to bortezomib treatment: Berthenet, K., Aimontche, E., et al.; Sci. Rep. 14, 3768 (2024), Abstract
- Nanobody-based retargeting of an oncolytic herpesvirus for eliminating CXCR4 + GBM cells: A proof of principle: J.S. Gil, et al.; Mol. Ther. Oncolytics 26, 35 (2022), Application(s): WB, Abstract
- Sex-dependent differences in the secretome of human endothelial cells.: Banfi, C., Brioschi, M., et al.; Biol. Sex Differ. 12, 7 (2021), Application(s): WB, Abstract
- Sex-dependent differences in the secretome of human endothelial cells: Banfi, C., Brioschi, M., et al.; Research Square , (2020), Application(s): WB
- Epigenetically Enhanced PDT Induces Significantly Higher Levels of Multiple Extrinsic Pathway Apoptotic Factors than Standard PDT, Resulting in Greater Extrinsic and Overall Apoptosis of Cutaneous T-cell Lymphoma: Salva, K. A., Kim, Y. H., et al.; Photochem. Photobiol. 94, 1058 (2018), Abstract
- Anticancer activities of epigallocatechin-3-gallate against cholangiocarcinoma cells.: Kim, H. J., Jeong, Y. I., et al.; Onco Targets Ther. 10, 137 (2017), Application(s): WB, Abstract
- Tamoxifen induces hepatotoxicity and changes to hepatocyte morphology at the early stage of endocrinotherapy in mice: F. Gao, et al.; Biomed Rep. 4, 102 (2016), Application(s): Western blot, Abstract — Full Text
- Targeting of BCL2 Family Proteins with ABT-199 and Homoharringtonine Reveals BCL2- and MCL1-Dependent Subgroups of Diffuse Large B-Cell Lymphoma: Klanova, M., Andera, L., et al.; Clin. Cancer Res. 22, 1138 (2016), Abstract
- Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL: J. Sun, et al.; Int. J. Clin. Exp. Pathol. 8, 6519 (2015), Application(s): Western blot , Abstract — Full Text
- Interleukin-24 inhibits influenza A virus replication in vitro through induction of toll-like receptor 3 dependent apoptosis: R. Weiss, et al.; Antiviral Res. 123, 93 (2015), Application(s): Western Blot, Abstract
- 2′-hydroxy c16-ceramide induces apoptosis-associated proteomic changes in c6 glioma cells: V. Kota, et al.; J. Proteome Res. 12, 4366 (2013), Abstract
- Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles: A.N. Böing, et al.; Biochim. Biophys. Acta 1833, 1844 (2013), Application(s): WB using transfected human breast carcinoma (MCF-7) cell lysates, Abstract
- Multiple effects of the Na+/H+ antiporter inhibitor HMA on cancer cells: F. Aredia, et al.; Apoptosis 18, 1586 (2013), Abstract
- Inhibition of vacuolar ATPase attenuates the TRAIL-induced activation of caspase-8 and modulates the trafficking of TRAIL receptosomes: V. Horova, et al.; FEBS J. 280, 3436 (2013), Abstract
- Chondroitin sulphate enhances the antitumor activity of gemcitabine and mitomycin-C in bladder cancer cells with different mechanisms: M. Ferro, et al.; Oncol. Rep. 27, 409 (2012), Application(s): WB using human bladder carcinoma (HT-1376) cell lysate, Abstract — Full Text
- A possible cross-talk between autophagy and apoptosis in generating an immune response in melanoma: A. Hossain, et al.; Apoptosis 17, 1066 (2012), Application(s): WB using human melanoma cell lysates, Abstract — Full Text
- NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy: I. Coupienne, et al.; Biochem. Pharmacol. 81, 606 (2011), Application(s): WB, Abstract
- RIP3 expression induces a death profile change in U2OS osteosarcoma cells after 5-ALA-PDT: I. Coupienne, et al.; Lasers Surg. Med. 43, 557 (2011), Abstract
- Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation: D. Xu, et al.; J. Biol. Chem. 286, 18261 (2011), Abstract
- Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts: K. Fernandez, et al.; Am. J. Pathol. 176, 416 (2010), Abstract
- The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Application(s): WB using human follicular lymphoma lysate, Abstract — Full Text
- Oncolytic adenoviral mutants induce a novel mode of programmed cell death in ovarian cancer.: Aerts, J. L., McNeish, I. A., et al.; Oncogene 27, 3081 (2008), Application(s): WB, Abstract
- Protein expression profiling and molecular classification of gastric cancer by the tissue array method: H.S. Lee, et al.; Clin. Cancer Res. 13, 4154 (2007), Abstract
- The histone deacetylase inhibitors depsipeptide and MS-275, enhance TRAIL gene therapy of LNCaP prostate cancer cells without adverse effects in normal prostate epithelial cells: Kasman, L., Lu, P., et al.; Cancer Gene Ther. 14, 327 (2007), Abstract
- S100A4 contributes to the suppression of BNIP3 expression, chemoresistance, and inhibition of apoptosis in pancreatic cancer: Mahon, P. C., Baril, P., et al.; Cancer Res. 67, 6786 (2007), Abstract
- Sphingosine-induced apoptosis in rhabdomyosarcoma cell lines is dependent on pre-mitochondrial Bax activation and post-mitochondrial caspases: Phillips, D. C., Martin, S., et al.; Cancer Res. 67, 756 (2007), Abstract
- Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles: W. Jian, et al.; Free Radic. Biol. Med. 39, 1162 (2005), Abstract
- Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma: P.C. Chiang, et al.; J. Hepatol. 43, 679 (2005), Abstract
- Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells: F. Jin, et al.; Cancer Res. 65, 6354 (2005), Abstract
- Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence: C. Douarre, et al.; Nucl. Acids Res. 33, 2192 (2005), Abstract — Full Text
- Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells: J.C. Lee, et al.; Carcinogenesis 26, 1716 (2005), Abstract
- Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells: C.H. Tang, et al.; FEBS Lett. 579, 265 (2005), Abstract
- Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation: L.F. Jarskog, et al.; Am. J. Psychiatry 161, 109 (2004), Abstract
- Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3: Y.H. Wu, et al.; J. Biol. Chem. 279, 19264 (2004), Abstract — Full Text
- Hydroxylation at C4’ or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species: C.H. Ko, et al.; Free Radic. Biol. 36, 897 (2004), Abstract
- Reversal of Taxol resistance in hepatoma by cyclosporin A: involvement of the PI-3 kinase-AKT 1 pathway: H.L. Lin, et al.; Br. J. Cancer 88, 973 (2003), Abstract
- Identification of a novel synthetic triterpenoid, methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate, that potently induces caspase-mediated apoptosis in human lung cancer cells: K.B. Kim, et al.; Mol. Cancer Ther. 1, 177 (2002), Abstract — Full Text
- Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production: Y.C. Chen, et al.; Biochem. Pharmacol. 64, 1713 (2002), Abstract
- Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat: W.R. Zhang, et al.; Brain Res. 947, 140 (2002), Abstract
- The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells: S.Y. Sun, et al.; Cancer Res. 62, 2430 (2002), Abstract — Full Text
- Identification of retinamides that are more potent than N-(4-hydroxyphenyl)retinamide in inhibiting growth and inducing apoptosis of human head and neck and lung cancer cells: S.Y. Sun, et al.; Cancer Epidemiol. Biomarkers Prev. 10, 595 (2001), Abstract — Full Text
- Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax: S.M. Cuddeback, et al.; J. Biol. Chem. 276, 20559 (2001), Abstract — Full Text
- The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change: H. Yamaguchi and H.G. Wang; Oncogene 20, 7779 (2001), Abstract
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