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Caspase-2 fluorometric assay kit

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Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

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Application	Activity assay, Fluorescent detection
Contents	25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl VDVAD-AFC Substrate (1mM), 100µl DTT.
Technical Info / Product Notes	VDVAD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity. Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
UniProt ID	P42575

Handling	Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2: Imre, G., Berthelet, J., et al.; EMBO Rep. 18, 733 (2017), Abstract

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