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Caspase-1 polyclonal antibody

ALX-210-804

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This antibody is covered by our Worry-Free Guarantee.

Caspase-3 polyclonal antibody Western blot — **Figure:** Western blot analysis of caspase-1 expression in rat liver tissue lysate using Caspase-1, pAb (Prod. No. ALX-210-804).”””,Caspase-1 polyclonal antibody Western blot,Caspase-1 polyclonal antibody Western blot,yes
ALX-210-806,210-806.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-806.gif,**Figure:** Western blot analysis of human caspase-3 using PAb to caspase-3 (Prod. No. ALX-210-806) with (lane 1) and without (lane 2) blocking peptide.”””””

Caspase-1 polyclonal antibody Immunohistochemistry — Immunohistochemistry staining of human lung tumor tissue using Caspase-1, pAb (Prod. no. ALX-210-804) at a 1:50 dilution showed positive staining in both cytoplasm and nucleus of lung epithelial cells. Images were taken at magnification of 20 x (A) and 63 x (B).

Product Details

Alternative Name	IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE
Application	ICC, IHC, IP, WB
Application Notes	Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5mM EDTA, and 0.03% Proclin.
Host	Rabbit
Immunogen	Synthetic peptide corresponding to aa 224-242 (CEPH224KTSDSTFLVFMSHGIREG242) of human caspase-1.
Purity Detail	Epitope-affinity purified.
Recommendation Dilutions/Conditions	Immunocytochemistry (10-20µg/ml)Immunohistochemistry (1:50)Immunoprecipitation (4-8µg/ml)Western Blot (0.5-4µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human, Mouse, Rat
UniProt ID	P29466
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer.: Lin, C. N., Liang, Y. L., et al.; J. Biomed. Sci. 31, 62 (2024), Application(s): WB, Abstract
The Inhibition of Autophagy and Pyroptosis by an Ethanol Extract of Nelumbo nucifera Leaf Contributes to the Amelioration of Dexamethasone-Induced Muscle Atrophy.: Park, E., Choi, H., et al.; Nutrients 15, (2023), Application(s): WB / Reactant(s): Mouse, Abstract
Lysophosphatidic Acid Induces Podocyte Pyroptosis in Diabetic Nephropathy by an Increase of Egr1 Expression via Downregulation of EzH2.: Kim, D., Ban, K. Y., et al.; Int. J. Mol. Sci. 24, (2023), Application(s): WB, Abstract
Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span: Kaverina, N., Schweickart, R. A., et al.; Aging (Albany NY) 15, 6658 (2023), Abstract
Methods to Study Inflammasome Activation in the Central Nervous System: Immunoblotting and Immunohistochemistry: Cyr, B., de Rivero Vaccari, J. P., et al.; Methods Mol. Biol. 2696, 223 (2023), Abstract
TGF-β activates NLRP3 inflammasome by an autocrine production of TGF-β in LX-2 human hepatic stellate cells.: Jun, H. S., Seo, E., et al.; Mol. Cell. Biochem. 477, 1329 (2022), Application(s): WB / Reactant(s): Human, Abstract
Protein-Bound Uremic Toxins Induce Reactive Oxygen Species-Dependent and Inflammasome-Mediated IL-1β Production in Kidney Proximal Tubule Cells: M. Mihajlovic, et al.; Biomedicines 9, 1326 (2021), Abstract
TGF-β activates NLRP3 Inflammasome by an Autocrine Production of TGF-β in LX-2 Human Hepatic Stellate Cells: Seo, E., Oh, Y. S., et al.; Research Square , (2021), Application(s): WB / Reactant(s): Human
Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction.: El-Remessy, A. B., Lemtalsi, T., et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1864, 746 (2018), Reactant(s): Mouse, Abstract
Preventive effect of Daiokanzoto (TJ-84) on 5-fluorouracil-induced human gingival cell death through the inhibition of reactive oxygen species production.: Yoshida, K., Okamura, H., et al.; PLoS One 9, e112689 (2014), Application(s): WB / Reactant(s): Human, Abstract

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Last modified: May 29, 2024

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Caspase-1 polyclonal antibody

ALX-210-804

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Caspase-1 polyclonal antibody

ALX-210-804

Product Details

Handling & Storage

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Datasheet, Manuals, SDS & CofA

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