carba-NAD⁺

carba-NAD⁺ is a NAD⁺ mimic with improved hydrolytic stability against adenosine diphosphate ribosyl transferase (ART) enzymes and NAD glycohydrolases. carba-NAD⁺ was shown to inhibit activity of the cholera toxin A ART enzyme and NAD⁺-consuming enzymes Sirt3/5, but was ineffective against PARP1 and NAD⁺-glycohydrolase. carba-NAD⁺ is a substrate of NAD⁺-handling enzymes like alcohol dehydrogenases and is suitable as hydrolysis-resistant NAD⁺-mimic for protein crystallizations. Robust cofactor for enzyme-based glucose sensing with glucose dehydrogenases and fluorescence lifetime detection.