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Calcineurin Phosphatase assay kit

BML-AK804

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Designed for inhibitor screening or kinetic assay of recombinant Calcineurin

Safe and non-radioactive assay
Convenient 1-step detection, no mixing needed
High throughput with 96 well plate format

This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

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Product Details

Application	Activity assay, Colorimetric detection, HTS
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	Recombinant calcineurin, RII phosphopeptide substrate, calmodulin, assay buffer, BIOMOL^® GREEN detection reagent, phosphate standard, microplate and detailed instructions.

Handling & Storage

Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Harmine and Kaempferol treatment enhances NFATC1 and FOXP3 mediated regulatory T-cells’ suppressive capacity in generalized vitiligo: P.S. Giri, et al.; Int. Immunopharmacol. 125, 111174 (2023), Abstract
The optimized core peptide derived from CABIN1 efficiently inhibits calcineurin-mediated T-cell activation: S. Lee, et al.; Exp. Mol. Med. 54, 613 (2022), Abstract
The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells: A. Rahman, et al.; Int. J. Stem. Cells , (2022), Abstract
A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line: S. Uesugi, et al.; Biosci. Biotechnol. Biochem. 85, 2153 (2021), Abstract
Endoplasmic Reticulum Stress Predicts Clinical Response to Cyclosporine Treatment in Primary Membranous Nephropathy: J. Tao, et al.; Am. J. Nephrol. 43, 348 (2016), Abstract
Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway: S. Xie, et al.; Arch. Biochem. Biopshys. 9861, 30224 (2016), Application(s): Harvested treated cardiomyocytes for calcineurin activity measurement, Abstract
CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation: R.R. Racine, et al.; Biochem. Biophys. Rep. 6, 172 (2016), Abstract — Full Text
Amoebae-Based Screening Reveals a Novel Family of Compounds Restricting Intracellular Legionella pneumophila: C.F. Harrison, et al.; ACS Infect. Dis. 1, 327 (2015), Abstract
Zinc-binding metallothioneins are key modulators of IL-4 production by basophils: T. Ugajin, et al.; Mol. Immunol. 66, 180 (2015), Application(s): Assay using mouse supernatant, Abstract
Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity: N. Ito, et al.; Biosci. Biotechnol. Biochem. 77, 954 (2013), Application(s): Rat and bovine brain samples analyzed by assay, Abstract — Full Text