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Sequence is calcineurin (CaN) autoinhibitory domain (rat brain CaN aa 457-482). It inhibits calcineurin with an IC50 of 10 µM using [32P]myosin light chain as substrate. Inhibits Mn2+-stimulated calcineurin activity but has no effect on Ni2+-stimulated activity. Ki=4.4 µM with radiolabeled [Ala97]-RII (81-99) peptide as the phosphatase substrate.
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| Regulatory Status |
RUO – Research Use Only |
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