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Calcineurin (human), (recombinant)

BML-SE163

8 citations

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Calcineurin (human), (recombinant) SDS-PAGE — SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 μg of purified human recombinant calcineurin protein (SE-163).

Product Details

Alternative Name	Protein phosphatase 2B, PP2B
Application Notes	Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates, and for screening inhibitors. Enzyme activity may be determined using the BIOMOL GREEN Calcineurin Assay Kit (Prod. No. BML-AK804).
Concentration	100 U/µl
Formulation	Liquid. In 1X KI-128 Assay Buffer with increased DTT concentration (50mM TRIS, pH 7.5, containing 100mM sodium chloride, 6mM MgCl₂, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl₂).
MW	60 kDa (calcineurin Aα) + 15 kDa (calcineurin B)
Purity	≥90% (SDS-PAGE)
Source	Produced in E. coli. Human calcineurin Aα (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosphatase 2B) is N-myristoylated on the CaNB subunit, similar to the native protein.
Specific Activity	One U= 1pmol/min using 0.15mM RII phosphopeptide substrate (Prod. No. BML-P160) in the presence of calcium/calmodulin at 30°C. In the absence of calmodulin, the activity is <1%.
UniProt ID	Q08209 (subunit Aα), P63098 (subunit Bα)

Handling & Storage

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Development of a monoclonal antibody specific for a calpain-generated Δ48 kDa calcineurin fragment, a marker of distressed astrocytes: S.D. Kraner, et al.; J. Neurosci. Methods 402, 110012 (2024), Abstract
A novel research model for evaluating sunscreen protection in the UV-A1: S.A. Figueiredo, et al.; J. Photochem. Photobiol. B. 178, 61 (2018), Abstract
Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast: R. Martin-Garcia, et al.; Cell Rep. 25, 772 (2018), Abstract
The phosphatase calcineurin regulates pathological TDP-43 phosphorylation: N.F. Liachko, et al.; Acta Neuropathol. 4, 545 (2016), Abstract
Ulinastatin attenuates neuropathic pain induced by L5-VRT via the calcineurin/IL-10 pathway: Ouyang, H., Nie, B., et al.; Mol. Pain 12, (2016), Abstract
Protein kinase C gamma-mediated phosphorylation of GluA1 in the postsynaptic density of spinal dorsal horn neurons accompanies neuropathic pain, and dephosphorylation by calcineurin is associated with prolonged analgesia: G. Miletic, et al.; Pain 156, 2514 (2015), Application(s): Intrathecal injection, Abstract — Full Text
Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB: D.L. Medina, et al.; Nat. Cell. Biol. 17, 288 (2015), Application(s): Assay, Abstract
The Ca2+-dependent phosphatase calcineurin controls the formation of the Carma1-Bcl10-Malt1 complex during T cell receptor-induced NF-kappaB activation: Palkowitsch, L., Marienfeld, U., et al.; J. Biol. Chem. 286, 7522 (2011), Abstract