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c-FLIP polyclonal antibody

ADI-AAP-440

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The cellular FLICE-like inhibitory protein, cFLIP, is a cellular homologue of the viral protein, vFLIP. cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively. cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligimerization of the adapter protein, FADD. cFLIP interacts with FADD and caspase-8 through their respective DEDs.

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c-FLIP polyclonal antibody Immunocytochemistry — Immunocytochemistry analysis of FLIP in HeLa cells with FLIP antibody at 5µg/ml.

c-FLIP polyclonal antibody Western blot — Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2µg/ml.

Product Details

Alternative Name	CASPER, CLARP, I-FLICE, Usurpin
Application	ICC, WB
Application Notes	Detects a band of ~28-58kDa by Western blot.
Formulation	Liquid. In PBS containing 0.02% sodium azide.
GenBank ID	U97074, U97075
Host	Rabbit
Immunogen	FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.
Purity Detail	Peptide affinity purified.
Recommendation Dilutions/Conditions	Immunocytochemistry (5µg/ml)Western Blot (0.5-1.0µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human, Mouse, Rat
UniProt ID	O15519
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Downregulation of c‑FLIP and upregulation of DR‑5 by cantharidin sensitizes TRAIL‑mediated apoptosis in prostate cancer cells via autophagy flux.: Nazim, U. M., Yin, H., et al.; Int. J. Mol. Med. 46, 280 (2020), Application(s): WB / Reactant(s): Human, Abstract
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell 73, 413 (2019), Abstract — Full Text
The transcription factor SP3 drives TNF-α expression in response to Smac mimetics: Beug, S. T., Cheung, H. H., et al.; Sci. Signal. 12, (2019), Abstract
Involvement of Up-Regulation of DR5 Expression and Down-Regulation of c-FLIP in Niclosamide-Mediated TRAIL Sensitization in Human Renal Carcinoma Caki Cells: J.M. Yun, et al.; Molecules 23, E2264 (2018), Abstract — Full Text
Codium fragile F2 sensitize colorectal cancer cells to TRAIL-induced apoptosis via c-FLIP ubiquitination: S.H. Park, et al.; Biochem. Biophys. Res. Commun. 508, 1 (2018), Abstract
Ophiopogonin B sensitizes TRAIL-induced apoptosis through activation of autophagy flux and downregulates cellular FLICE-like inhibitory protein: U.M. Nazim, et al.; Oncotarget 9, 4161 (2017), Abstract — Full Text
The coffee diterpene kahweol enhances sensitivity to sorafenib in human renal carcinoma Caki cells through down-regulation of Mcl-1 and c-FLIP expression: K.J. Min, et al.; Oncotarget. 8, 83195 (2017), Abstract — Full Text
Oncolytic virus synergizes with Smac mimetic compounds to induce rhabdomyosarcoma cell death in a syngeneic murine model.: Holcik, M., Korneluk, R. G., et al.; Oncotarget 8, 3495 (2017), Application(s): WB, Abstract
Inhibitor of Apoptosis Protein-1 Regulates Tumor Necrosis Factor-Mediated Destruction of Intestinal Epithelial Cells: Grabinger, T., Bode, K. J., et al.; Gastroenterology 152, 867 (2017), Abstract
PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP: Wang, H., Meng, H., et al.; PNAS 114, 11944 (2017), Abstract

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c-FLIP polyclonal antibody

ADI-AAP-440

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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