Boc-Ala-Ala-Nva-SBzl

Much more sensitive substrate for leukocyte proteinase 3 (PR-3, myeloblastin) (K_cat/k_m=1.06 x 10⁶ M^-1s^-1, K_m=63 µM) than MeOSuc-AAPV-pNA (Chromogenic Substrate) (Prod. No. BML-P213) (K_cat/k_m=1.06 x 10³ M^-1s^-1, K_m=470 µM). Also cleaved by neutrophil elastase (K_cat/k_m=9.2 x 10⁶ M^-1s^-1, K_m=2.2 µM), granzyme H, cathepsin G, chymotrypsin, and rat MCPI and II. Typical assay conditions: substrate concentration 88-250 µM; enzyme concentration 0.2-20 nM; buffer [100 mM HEPES pH 7.5, 500 mM NaCl, 10% DMSO]; with 170 µM either 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) or 4,4′-dithiobis(pyridine) (from 15 mM DMSO stocks); 25°C. Hydrolysis of substrate can be detected at 412 nm for DTNB (