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Blebbistatin(+/-)

BML-EI315

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Inhibitor of non-muscle myosin II ATPase

Rapid and selective inhibitor of Myosin II ATPase
Used in cardiovascular research
Highly cited

Blebbistatin (so named because of its ability to block cell blebbing) is a rapid, selective, and high-affinity (IC₅₀ approx. 4 µM) inhibitor of non-muscle myosin II ATPase activity. During cell division, blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. Blebbistatin impedes cell migration and cytokinesis. Blebbistatin also reduces infiltration of inflammatory cells, inhibits pancreatic adenocarcinoma cellular invasiveness, PDGF-stimulated fibroblast-collagen matrix contraction, and diminishes harm to vascular system in models of arterial thrombosis in vivo.

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Product Details

Alternative Name	(±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one, (±)-Blebbistatin
Appearance	Bright yellow solid.
CAS	674289-55-5
Couple Target	Myosin
Couple Type	Inhibitor
Formula	C₁₈H₁₆N₂O₂
Identity	Identity determined by NMR.
MW	292.3
Purity	≥97% (HPLC)
Solubility	Soluble in DMSO (25mg/ml).

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Exposure to blue light (i.e. 450-490nm) may lead to the degradation and inactivation of blebbistatin via cytotoxic intermediates, which may be a source of concern when used in fluorescence applications.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells: Ishihara, S., Enomoto, A., et al.; iScience 28, 112057 (2025), Abstract
Insulin mitigates acute ischemia-induced atrial fibrillation and sinoatrial node dysfunction ex vivo: Qiu, H., Li, F., et al.; JCI Insight 10, (2024), Abstract
Synchronizing systolic calcium release with azumolene in an experimental model: D. Si, et al.; Heart Rhythm O2 3, 568 (2022), Abstract
Statins Protect Against Early Stages of Doxorubicin-induced Cardiotoxicity Through the Regulation of Akt Signaling and SERCA2: K. Dadson, et al.; CJC open 4, 1043 (2022), Abstract
Probing TGF-β1-induced cytoskeletal rearrangement by fluorescent-labeled silica nanoparticle uptake assay: H.R. Shin, et al.; Biochem. Biophys. Rep. 28, 101137 (2021), Abstract
LFA-1 and Kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors: K.P. Eidell, et al.; J. Cell Sci. 36, 258602 (2021), Abstract
Anti-arrhythmic and inotropic effects of empagliflozin following myocardial ischemia: M.A. Azam, eta al.; Life Sci. 276, 119440 (2021), Abstract
Osmotic gradients induce stable dome morphogenesis on extracellular matrix: S. Ishida-Ishihara, et al.; J. Cell Sci. 133, jcs243865 (2020), Abstract
Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export: C. Bruyere, et al.; Sci. Rep. 9, 15565 (2019), Abstract — Full Text
Omegasome-proximal PtdIns(4,5)P2 couples F-actin mediated mitoaggregate disassembly with autophagosome formation during mitophagy: C.W. Hsieh, et al.; Nat. Commun. 10, 969 (2019), Application(s): added during cell culture to screen for functional and structrual changes in mitophagy in HeLa cells expressing EBFP-2-Parkin, EGFP-dMito and KillerRed-dMito, Abstract — Full Text

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Blebbistatin(+/-)

BML-EI315

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Blebbistatin(+/-)

BML-EI315

Product Details

Handling & Storage

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