BIOARRAY™ Single-round RNA amplification and biotin labeling system

• Maintains the value of legacy data by the continued use of Enzo kit, the gold standard  for GeneChip^® visualization.
• Decreases experimental variation and standardizes biological data derived from microarrays with the universally accepted system.
• Maximizes incorporation efficiency and produces strong, clear displays by utilizing two biotin-labeled nucleotides and a high quality T7 polymerase.
• Convenient workflow with a flexible 4-16 hour transcription time and reagents supplied in a ready-to-use format.
• Enables correlation of results from experiment-to-experiment, project-to-project and lab-to-lab.

The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 250 ng and as high as 5000 ng. The higher yield results from an improved cDNA purification system, now provided with the kit.

The Single-Round RNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and purification and in vitro transcription labeling.

Note: Control RNA (100µg/ml) (Prod. no. ENZ-42406-CR) is available separately.